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Two conditions experiment, ChIP-chip anti-Sir3 comparing Sir3 occupancy in exponentially growing S.cerevisiae cells to Sir3 occupancy in the dense fraction of 7 days YPD culture of S.cerevisiae Two conditions experiment with biological duplicates. Both conditions were processed on the same array, du...
ORGANISM(S): Saccharomyces cerevisiae 
The main objective of this project is the characterization of cell-to-cell transcriptional heterogeneity in and out of the yeast quiescent state. Do quiescent cells manage to respond rapidly to their environment through a general transcriptional permissiveness, or through a cell- to-cell heterogenei...
ORGANISM(S): Saccharomyces cerevisiae 
Gradient-enriched quiescent cells of a 6 days stationary phase culture were refed with rich medium (Yeast Peptone Dextrose 2%) in the presence or absence of RNAPII inhibition by various means (rpb1-1 thermosensitive mutant, thiolutin). Samples were harvested in quiescence and 30 minutes post-refeedi...
ORGANISM(S): Saccharomyces cerevisiae 
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