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OfMasc-GFP derivatives or GFP were transiently expressed in OfTC cells. At 2 days post transfection, OfTC cells were treated with 100 nM Bortezomib for 1 day. The OfT1C cells were then fixed with 0.1% formaldehyde, and the fixation was quenched with 300 mM glycine. The cells were lysed on ice in 1 m...
ORGANISM(S): Cellular Organisms 
To reveal comprehensive gene expression profiles and their characteristics of the rice egg and synergid cell, we performed cell type-specific microarray analysis using nonenzymatic cell isolation technique. 40 and 50 genes expressed specifically in the egg cell and synergid cell were identified by c...
ORGANISM(S): Oryza sativa 
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