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Chromosomes and genes are non-randomly arranged within the mammalian cell nucleus. Clustering of genes is of great significance in transcriptional regulation. However, the relevance of gene clustering in their expression during differentiation of neural precursor cells (NPCs) into astrocytes remains...
ORGANISM(S): Mus musculus 
For immunoprecipitation to detect the endogenous interaction between VGLL3 and EWSR1, cardiac myofibroblasts were fixed with 0.1% PFA and lysed in IP buffer [20 mM HEPES­NaOH (pH 7.5)/1 mM EGTA/1 mM MgCl2/150 mM NaCl /5% glycerol/1% NP­40] containing protease inhibitors (1:100) and phosphatase inhib...
ORGANISM(S): Mus Musculus (mouse) 
For immunoprecipitation to detect the endogenous interaction between VGLL3 and DDX5, cardiac myofibroblasts were fixed with 0.1% PFA and lysed in IP buffer [20 mM HEPES­NaOH (pH 7.5)/1 mM EGTA/1 mM MgCl2/150 mM NaCl /5% glycerol/1% NP­40] containing protease inhibitors (1:100) and phosphatase inhibi...
ORGANISM(S): Mus Musculus (mouse) 
Cardiac myofibroblasts retrovirally transduced with FLAG-VGLL3 or control vector were fixed with 0.1% PFA and lysed in a HEPES-RIPA buffer (20 mM HEPES-NaOH [pH 7.5]/1 mM EGTA/1 mM MgCl2/150 mM NaCl/0.25% sodium deoxycholate/0.05% SDS/1% NP-40) containing protease inhibitors (1:100), phosphatase inh...
ORGANISM(S): Mus Musculus (mouse) 
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