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UnknownEnzalutamide versus flutamide for castration-resistant prostate cancer after combined androgen blockade therapy with bicalutamide: study protocol for a multicenter randomized phase II trial (the OCUU-CRPC study).
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S-EPMC6458677 | biostudies-literature
Proteomics100uM_DSBU_XL-MS_PHB1-Myc_HEK293
Mitochondria were isolated from HEK293 cells that stably express PHB1 with an C-terminal 3×Myc tag, and were resuspended in a cross-linking buffer [1 × PBS (pH 7.2) and 100 μM disuccinimidyl didutyric urea (DSBU) for 30 min at room temperature. The cross-linked mitochondria were then lysed in RIPA b...
ORGANISM(S): Homo Sapiens (human) 
2019-09-05 | PXD014217 | JPOST Repository
ProteomicsBioID2_BirA-MAVS_CLPB-BirA_PHB1-BirA_HEK293
HEK293 cells that stably express MAVS, CLPB or PHB1 fused at C-terminus with the biotin ligase from A. aeolicus were incubated with 100 μM biotin for 18 h. The mitochondrial fractions were lysed in RIPA buffer, and the clarified supernatants were incubated with streptavidin agarose beads for 2 h at ...
ORGANISM(S): Homo Sapiens (human) 
2019-09-05 | PXD014218 | JPOST Repository
Proteomics50_100uM_DSBU_XL-MS_PHB2(V1)-Myc_HEK293
Mitochondria were isolated from HEK293 cells that stably express PHB2(V1) with an C-terminal 3×Myc tag, and were resuspended in a cross-linking buffer [1 × PBS (pH 7.2) and 50 or 100 μM disuccinimidyl didutyric urea (DSBU) for 30 min at room temperature. The cross-linked mitochondria were then lysed...
ORGANISM(S): Homo Sapiens (human) 
2019-09-05 | PXD011941 | JPOST Repository
ProteomicsBioID2_PHB2(V1)_PHB2(V3)-BirA-HA_HEK293
HEK293 cells that stably express PHB2 (V1 or V3) fused at C-terminus with the biotin ligase from A. aeolicus were incubated with 100 μM biotin for 18 h. The mitochondrial fractions were lysed in RIPA buffer, and the clarified supernatants were incubated with streptavidin agarose beads for 2 h at 4° ...
ORGANISM(S): Homo Sapiens (human) 
2019-09-05 | PXD011939 | JPOST Repository
ProteomicsBioID2_PHB2(V1)-(WT_KKK_I225P)
HEK293 cells that stably express wild-type (WT), E229/231/233K (KKK), or I225P mutant PHB2(V1) fused at C-terminus with the biotin ligase from A. aeolicus were incubated with 100 μM biotin for 18 h. The mitochondrial fractions were lysed in RIPA buffer, and the clarified supernatants were incubated ...
ORGANISM(S): Homo Sapiens (human) 
2019-09-05 | PXD011946 | JPOST Repository
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