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ProteomicsAnalysis of proteins in supernatant and precipitate fractions of human bronchial smooth muscle cells by 1D SDS-PAGE and quantitative LC-MS/MS
Supernatant and precipitate protein fractions of human bronchial smooth muscle cells (HBSMC) after sonication and centrifugation were analyzed using 1D SDS-PAGE. The gel lanes were cube-cut into thirty-three 1.1 mm x 1.1 mm x 1 mm (gel thickness) squares and each gel piece was subjected to in-gel d...
ORGANISM(S): Homo sapiens (Human) 
2019-01-03 | PXD010036 | Pride
Precipitation fraction 1d sds-page Supernatant fraction Bronchial smooth muscle cells Quantitative lc-ms/ms 
UnknownNetwork Pharmacology and Experimental Validation to Explore the Mechanism of Qing-Jin-Hua-Tan-Decoction Against Acute Lung Injury.
Not available
S-EPMC9304690 | biostudies-literature
UnknownSuppressing inflammatory signals and apoptosis-linked sphingolipid metabolism underlies therapeutic potential of Qing-Jin-Hua-Tan decoction against chronic obstructive pulmonary disease.
Not available
S-EPMC10839876 | biostudies-literature
ProteomicsAnalysis of LDL-associated proteins using the method of digitized native protein mapping
The method of digitized native protein mapping, combining nondenaturing micro 2DE, grid gel-cutting and quantitative LC-MS/MS (in data-independent acquisition mode, or MSE), was improved by using a new MS/MS mode, ion mobility separation enhanced-MSE (HDMSE) and applied to analyze the area of human ...
ORGANISM(S): Homo sapiens (Human) 
2016-05-17 | PXD003720 | Pride
Grid gel-cutting Quantitative lc-hdmse Human plasma low-density lipoprotein Protein-protein interactions Nondenaturing micro 2de Digitized native protein mapping 
UnknownExhausting T Cells During HIV Infection May Improve the Prognosis of Patients with COVID-19.
Not available
S-EPMC8502810 | biostudies-literature
MetabolomicsTranscriptomics and metabolomics of engineered Synechococcus elongatus during photomixotrophic growth

BACKGROUND: Converting carbon dioxide (CO2) into value-added chemicals using engineered cyanobacteria is a promising strategy to tackle the global warming and energy shortage issues. However, most cyanobacteria are autotrophic and use CO2 as a sole carbon source, which makes it h...

2022-03-21 | MTBLS2825 | MetaboLights
ProteomicsProteomic analysis of cellular soluble proteins from human bronchial smooth muscle cells by combining nondenaturing micro 2DE and quantitative LC-MS/MS: Preparation of more than 4000 native protein maps
Soluble proteins of human bronchial smooth muscle cells (HBSMC) were separated by nondenaturing micro 2DE and a 30 mm X 40 mm area of the CBB-stained slab gel (1.0 mm thick) was cut into 1.1 mm X 1.1 mm squares, then the proteins in the 972 gel pieces (squares) were applied to quantitative LC-MS/MS....
ORGANISM(S): Homo sapiens (Human) 
2015-08-10 | PXD001471 | Pride
Cellular proteins Native protein map Nondenaturing micro 2de Lc-ms/ms Quantitative protein-protein interaction 
UnknownClinical course and outcomes of critically ill patients with SARS-CoV-2 pneumonia in Wuhan, China: a single-centered, retrospective, observational study.
Not available
S-EPMC7102538 | biostudies-literature
UnknownAbnormal Coagulation Function of Patients With COVID-19 Is Significantly Related to Hypocalcemia and Severe Inflammation.
Not available
S-EPMC8242574 | biostudies-literature
UnknownHIF2α drives ccRCC metastasis through transcriptional activation of methylation-controlled J protein and enhanced prolegumain secretion.
Not available
S-EPMC11825665 | biostudies-literature
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