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TranscriptomicsTranscription profiling of human B cell subtypes derived from peripheral blood or umbilical cord blood.
Goals/objectives: to identify various gene expression in B cell subsets derived from human PBMC and cord blood Sample: human PBMC and human cord blood Cell subsets: memory B cell (CD20+CD10-CD27+), naive B cell (CD20+CD10-CD27-), CD21lo transitional B cell (CD20+CD10+CD27-CD21lo), CD21hi transitio...
ORGANISM(S): Homo sapiens 
2010-04-12 | E-GEOD-17186 | biostudies-arrayexpress
TranscriptomicsHuman peripheral blood CD4+ T cell subsets
Cells were isolated from healthy human donors (n=2). Unstimulated cells. Cells were stained with CD4, CD45RA, CCR7 and CXCR7. Using flow cytometry, 4 CD4+ T cell populations were sorted: (1) Naïve (CD45RA+CCR7+CXCR5-), (2) Central memory (CD45RA-CCR7+CXCR5-), (3) Effector memory (CD45RA-CCR7-CXCR5-)...
ORGANISM(S): Homo sapiens 
2011-08-03 | E-GEOD-26928 | biostudies-arrayexpress
TranscriptomicsIdentification of IL-21-induced STAT3 dependent genes in human B cells
IL-21 induces B cell activation, and differentiation into antibody-secreting plasmablasts in vitro. This process is abolished by loss-of function mutations in STAT3 We used microarrays to identify genes that are induced by IL-21 in a STAT3-dependent manner Sort-purified naïve B cells from four heal...
ORGANISM(S): Homo sapiens 
2014-01-09 | E-GEOD-51587 | biostudies-arrayexpress
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