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Purpose: Investigate effects of high salt on human macrophage activation Methods: Human monocytes-derived macrophages were treated by additional 51mM NaCl for 24 hours (NaCl groups) or not (Control groups). mRNA profiles were generated by RNA-Seq, in triplicate, using Ion proton(Life tech). qRT–PCR ...
ORGANISM(S): Homo sapiens 
The cell intrinsic antiviral response of multicellular organisms developed over millions of years and critically relies on the ability to sense and eliminate viral nucleic acids. Here we used an affinity proteomics approach in evolutionary distant species (human, mouse and fly) to identify proteins ...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) Drosophila melanogaster (Fruit fly) 
2021-09-22 | PXD027894 | Pride
As a test for the effectiveness of SPIDER with RNA-protein pairs, we examined SARS-CoV-2 Nucleoprotein. We used Nucleoprotein and specific biotin-RNA to validate the efficiency of spider capturing N and analysised the pupylation sites of N protein by mass spectrometry
ORGANISM(S): Severe Acute Respiratory Syndrome Coronavirus 2 
2021-06-04 | PXD026518 |
Using ssRNA-seq, we examined the alteration of transcription profiles in the nucleus accumbens of methamphetamine-sensitized mice. Methamphetamine was a commonly abused psychostimulant. Repeated exposure to methamphetamine elicited long-lasting cellular and molecular changes, including the aberrant ...
ORGANISM(S): Mus musculus 
Using microRNA-Seq, we examined the alteration of microRNA expression profiles in the nucleus accumbens of methamphetamine-sensitized and saline-control mice. Nucleus accumbens lysate from eight mice of each group were pooled as one sample. Two RNA samples from each group were prepared and were then...
ORGANISM(S): Mus musculus 
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