293 stable cell line expressing NRBP1 or NRBP1 mutant were transfected with HA-TR-TUBE using TR-TUBE system to protect the degradation of the ubiquitin chains of the potential ligase substrate. The complexes were pulled down using anti-HA antibody and subsequently digested with modified Trypsin. Ant...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606)
