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Lungs were collected as soon as possible after thermal injury 30-40 mins post injury for neutrophil isolation. Lungs were minced in HBSS with 1% BSA (Sigma) and strained through a 40um nylon filter (BD). Samples were sorted using BD fusion machine. A 100um nozzle was used at a flow rate of 2.RNA wa...
ORGANISM(S): Mus musculus 
Based on the observations that influenza infection led to behavioral changes in alveolar macrophages (AMs), we performed an RNAseq experiment to delineate global gene expression changes that occurred in the AM population after influenza infection versus a control (non-infected) population.
ORGANISM(S): Mus musculus 
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