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ProteomicsIdentification of proteins and miRNAs that specifically bind an mRNA in vivo
Understanding regulation of an mRNA requires knowledge of its regulators. However, methods for reliable de-novo identification of proteins binding to a particular RNA are scarce and were so far only successfully applied to abundant noncoding RNAs in cell culture. Here, we present vIPR, an RNA-protei...
ORGANISM(S): Caenorhabditis elegans 
2019-08-12 | PXD013720 | Pride
Q exactive hf-x Caenorhabditis elegans Rna pulldown Q exactive plus 
TranscriptomicsUnstressed HeLa cells and ELAVL1/HuR knock down conditions: polyA RNA-Seq, small RNA-Seq, and PAR-CLIP
Post-transcriptional gene regulation relies on hundreds of RNA binding proteins (RBPs) but the function of most RBPs is unknown. The human RBP HuR/ELAVL1 is a conserved mRNA stability regulator. We used PAR-CLIP, a method based on RNA-protein crosslinking, to identify transcriptome wide ~26,000 HuR ...
ORGANISM(S): Homo sapiens 
2011-06-29 | E-GEOD-29943 | biostudies-arrayexpress
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