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Exome sequencing of high-risk prostate cancer
high-throughput sequencing of methylated and hydroxymethylated DNA from tumor and non-tumor tissue of patients with high-risk prostate cancer
Data Access Committee EGAC00001000260
DNA extracted from tumoral tissue of 39 patients was used for exome sequencing. As a comparison, DNA extracted from blood was used as a non-tumoral control. DNA was captured using the EZ SeqCap v3 exome kit from Roche and sequenced using an Illumina HiSeq instrument.
Immunoprecipitation of methylated and hydroxymethylated DNA was performed on DNA isolated from tumoral and non-tumoral prostate tissue of patients with high-risk disease. Samples were sequenced on the Illumina HiSeq as 50 bp single end.
Transcriptomic analysis using qRT-PCR was performed on fresh frozen clear-cell renal cell carcinoma tissues treated with VEGFR-TKIs in first line (n=109). The transcriptomic data from this experiment were analyzed together with miRNA sequencing data from FFPE samples from the same tumor (E-MTAB-1058...
ORGANISM(S): Homo sapiens 
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