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Programmable mRNA translation initiation by trans-RNA
StitchR: Ribozyme-activated mRNA Trans-ligation and Translation
Collided ribosomes are rescued by the endonuclease Rae1 and trans-translation [Ribo-Seq]
Collided ribosomes are rescued by the endonuclease Rae1 and trans-translation [RNA-Seq]
The ability to modulate specific gene expression is crucial for interrogating, perturbing, and engineering cellular systems. A wide range of approaches have been developed for gene silencing, but few tools are available to activate individual mRNAs for translation inside cells. 16Guiding ribosomes t...
ORGANISM(S): Homo sapiens Mus musculus 
2025-10-02 | GSE277746 | GEO
We report that ribozyme cleavage of two separate mRNAs activated their scarless trans-ligation and translation into full-length protein in eukaryotic cells, a process that we named StitchR (for stitch RNA).
ORGANISM(S): Homo sapiens 
2024-11-14 | GSE277847 | GEO
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