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TranscriptomicsGene expression analysis after reconstitution of Ebf1-deficient pre-pro-B cells with Ebf1
Ebf1 deficient pre-pro B-cells (Fraction A) can be cultured in the presence of stromal feeders and cytokines. The retroviral transduction of these cells with Ebf1 was used as gain-of-function experiment for the analysis of direct and functional target genes of Ebf1. Ebf1 deficient pre-pro B-cells we...
ORGANISM(S): Mus musculus 
2010-07-29 | E-GEOD-21455 | biostudies-arrayexpress
TranscriptomicsEbf1 ChIP
Ebf1 is a key determinant of B-lymphocyte specification. In order to identify unknown transcriptional targets, endogenous Ebf1 was isolated by chromatin-IP from primary pro-B cells and the copurified DNA was hybridized to promoter tiling arrays. Ebf1 was ChIPed using a polyclonal antibody directed a...
ORGANISM(S): Mus musculus 
2010-06-24 | E-GEOD-21469 | biostudies-arrayexpress
TranscriptomicsThe crystal structure of the NHL domain in complex with RNA reveals the molecular basis of Drosophila Brain tumor-mediated gene regulation
Here, we analyze the RNA-binding preferences of several NHL containing RNA-binding proteins (BRAT, NCL-1, TRIM71, WECH, LIN-41, TRIM56, and MEI-P26) using RNAcompete. In the RNAcompete assay, purified GST-tagged RNA-binding protein is incubated with an excess of RNA pool and bound RNA from individua...
ORGANISM(S): synthetic construct 
2015-09-14 | E-GEOD-73000 | biostudies-arrayexpress
TranscriptomicsPancreas-specific deletion of Ikba and RelA.
The NfkB-pathway is activated early during acute pancreatitis. We investigated the influence on gene expression of two pancreas-specific deletions interfering with NfkB-activation. Pancreata from 8 week old mice were prepared, RNA was isolated and Affymetrix microarray expression analysis was perfor...
ORGANISM(S): Mus musculus 
2012-12-02 | E-MTAB-886 | biostudies-arrayexpress
UnknownClopidogrel and Aspirin in Acute Ischemic Stroke and High-Risk TIA.
Not available
S-EPMC6193486 | biostudies-literature
UnknownIntensive vs Standard Treatment of Hyperglycemia and Functional Outcome in Patients With Acute Ischemic Stroke: The SHINE Randomized Clinical Trial.
Not available
S-EPMC6652154 | biostudies-literature
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