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TranscriptomicsProtease activated receptor 2 (PAR2) on HEK293 activated by trypsin or 2f-LIGRLO-NH2
Human embryonic kidney cell (HEK293) were treated with PAR2 peptide agonist 2f-LIGRLO-NH2 (1.5h, 3h, 6h and 12h) or trypsin (6h). Comparison of genes similarly regulated by both treatments allowed better characterization of PAR2 induced response as both agonists had been reported as non-specific for...
ORGANISM(S): Homo sapiens 
2010-11-19 | E-GEOD-24332 | biostudies-arrayexpress
GenomicsEnzymatic treatment using trypsin and cold-active protease for C2C12 myoblasts
Enzymatic treatment using trypsin and cold-active protease for C2C12 myoblasts
PRJNA707443 | ENA
TranscriptomicsThe effect of trypsin-2 on human tongue squamous cell carcinoma cell line gene expression
The level of trypsin-2 has been shown to correlate with the malignancy and metastatic potential of many cancer. We used microarrays to detail how tryspin-2 overexpression changes the gene profile of human tongue squamous cell carcinoma cell line (HSC-3). We compared trypsin-2 overexpressing HSC-3 li...
ORGANISM(S): Homo sapiens 
2012-10-02 | E-GEOD-32056 | biostudies-arrayexpress
TranscriptomicsSerine protease-mediated wounding identifies localized epidermal wound response genes in Drosophila melanogaster
We have used trypsin-mediated wounding to amplify the transcriptional response to clean puncture wounding. We studied the transcriptional response of clean puncture wounding and trypsin puncture wounding in relation to unwounded wild-type late-stage embryos. Trypsin puncture wounding generally incr...
ORGANISM(S): Drosophila melanogaster 
2013-08-12 | E-MEXP-3755 | biostudies-arrayexpress
TranscriptomicsGene expression profile of gliadin effects on intestinal epithelial cells suggests novel non-enzymatic functions of pepsin and trypsin
Gliadin triggers T-cell mediated immunity in celiac disease, and has cytotoxic effects on enterocytes mediated through obscure mechanisms. In addition, gliadin transport mechanisms, potential cell surface receptors and gliadin-activated downstream signaling pathways are not completely understood. In...
ORGANISM(S): Homo sapiens 
2013-03-21 | E-GEOD-45357 | biostudies-arrayexpress
TranscriptomicsEnzymatic treatment using trypsin and cold-active protease for C2C12 myoblasts
We have comprehensively profiled the time-series changes in gene expression dependent on enzymatic treatment using trypsin, and attempted to elucidate the global changes suppressed by treatment with cold active protease in a low-temperature environment.
ORGANISM(S): Mus musculus 
2022-06-20 | GSE168452 | GEO
ProteomicsAbsolute Quantification of Apolipoproteins Following Treatment with Omega-3 Carboxylic Acids and Fenofibrate Using a High Precision Stable Isotope-Labeled Recombinant Protein Fragments Based SRM Assay
Stable isotope-labeled standard (SIS) peptides are used as internal standards in targeted proteomics to provide robust protein quantification, which is required in clinical settings. However, SIS peptides are typically added post trypsin digestion and, as the digestion efficiency can vary significan...
ORGANISM(S): Homo Sapiens 
PXD013314 | panorama
ProteomicsQuantitative proteomic analysis of trypsin-treated extracellular vesicles to evaluate non-vesicular contaminants and surface vesicular proteins.
Extracellular vesicles (EVs) are lipid bilayered vesicles released into extracellular milieu by most of cells. Currently, various purification methods of EVs have been established but most of studies use ultracentrifugation-based purification of EVs. However, these methods have the problem of co-pur...
ORGANISM(S): Homo sapiens (Human) 
2021-08-30 | PXD001355 | Pride
Non-vesicular contaminants Quantitative proteomics Extracellular vesicles Trypsin treatment 
TranscriptomicsExpression data from mammary epithelial cells harvested from 8-9 week old C3H female mice - various isolation conditions
Numerous lineage analysis studies have used FACS sorting as a separation technique prior to measuring mRNA expression patterns. To assess if FACS sorting causes non-specific changes in gene expression, we collected a heterogeneous population of mammary epithelial cells from three biological replicat...
ORGANISM(S): Mus musculus 
2014-09-05 | E-GEOD-61167 | biostudies-arrayexpress
TranscriptomicsExpression data from mice on a high fat, high carbohydrate diet treated with exenatide
The present study was constructed to confirm previous findings that mice on a high fat diet (HFD) treated by subcutaneous injection with exenatide (EXE) at 3µg/kg once daily for 6 weeks develop exocrine pancreatic injury (Rouse et al. 2014). The present study included 12 weeks of EXE exposure at mu...
ORGANISM(S): Mus musculus 
2014-06-01 | E-GEOD-54774 | biostudies-arrayexpress
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