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TranscriptomicsTranswell-cultured and miRNAs-transfected T84 cells
MicroRNAs are small non-coding RNA species, some of which are playing important roles in cell differentiation. However, the level of participations of microRNAs in epithelial cell differentiation is largely unknown. Here, we found that expression levels of four microRNAs (miR-210, miR-338-3p, miR-33...
ORGANISM(S): Homo sapiens 
2009-07-14 | E-GEOD-15385 | biostudies-arrayexpress
TranscriptomicsKYSE170 transfected with ncRNA or miR-210
MicroRNAs are small non-coding RNA species, some of which are playing important roles in malignancies. However, roles of miR-210 in cancer have yet to be unknown and controversial. Here, we found the expression level of miR-210 is downregulated in human esophageal squamous cell carcinoma and its cel...
ORGANISM(S): Homo sapiens 
2011-01-05 | E-GEOD-20637 | biostudies-arrayexpress
TranscriptomicsExpression data of macrophage phenotypes treated with interleukin-1alpha
Identification of the difference in responsiveness to interleukin-1alpha between M1 and M2 macrophage phenotypes. To identify the difference in responsiveness to interleukin-1alpha between M1 and M2 macrophage phenotypes, we performed micorarray analysis of gene expression in two phenotypes with or ...
ORGANISM(S): Mus musculus 
2014-09-21 | E-GEOD-53680 | biostudies-arrayexpress
TranscriptomicsTrastuzumab produces therapeutic actions by upregulating miR-26a and miR-30b in breast cancer cells
Objective: Trastuzumab has been used for the treatment of HER2-positive breast cancer (BC). However, a subset of BC patients exhibited resistance to trastuzumab therapy. Thus, clarifying the molecular mechanism of trastuzumab treatment will be beneficial to improve the treatment of HER2-positive BC ...
ORGANISM(S): Homo sapiens 
2012-06-02 | E-GEOD-38415 | biostudies-arrayexpress
TranscriptomicsTranscriptome expressed in the mouse suprachiasmatic nucleus
This array set was used to identify the genes that are highly expressed in the mouse suprachiasmatic nucleus (SCN). Because pharmacological inhibition of Gai/o activity with pertussis toxin hampers intercellular synchronization and causes dampened rhythms of the entire SCN, we hypothesized that memb...
ORGANISM(S): Mus musculus 
2011-04-22 | E-GEOD-28574 | biostudies-arrayexpress
TranscriptomicsMicroRNA Profile Predicts Recurrence of Hepatocellular Carcinoma in Milan Criteria Cases with Mild Liver Cirrhosis.
In this study, we utilized microRNA expression profiling to assess risk of HCC recurrence after liver resection. We examined microRNA expression profiling in paired tumor and non-tumor liver tissues of 73 HCC patients with mild cirrhosis (Child-Pugh A/B) who satisfy Milan Criteria. We constructed pr...
ORGANISM(S): Homo sapiens 
2011-02-08 | E-GEOD-21362 | biostudies-arrayexpress
TranscriptomicsGene expression profiles of esophageal squamous cell carcinoma cell lines
Profiles of esophageal squamous cell carcinoma and normal esophageal normal epithelium normal cell line. Analysis provides validation of novel microRNA targets prediction algorithms. esophageal squamous cell carcinoma:14, normal epithelium cell:2
ORGANISM(S): Homo sapiens 
2016-09-01 | E-GEOD-23964 | biostudies-arrayexpress
ProteomicsImmunnoprecipitation followed by PRM to detect endogenous interaction between VGLL3 and DDX5
For immunoprecipitation to detect the endogenous interaction between VGLL3 and DDX5, cardiac myofibroblasts were fixed with 0.1% PFA and lysed in IP buffer [20 mM HEPES­NaOH (pH 7.5)/1 mM EGTA/1 mM MgCl2/150 mM NaCl /5% glycerol/1% NP­40] containing protease inhibitors (1:100) and phosphatase inhibi...
ORGANISM(S): Mus Musculus (mouse) 
2022-12-24 | PXD039093 | JPOST Repository
ProteomicsImmunoprecipitation followed by PRM to detect endogenous interaction between VGLL3 and EWSR1
For immunoprecipitation to detect the endogenous interaction between VGLL3 and EWSR1, cardiac myofibroblasts were fixed with 0.1% PFA and lysed in IP buffer [20 mM HEPES­NaOH (pH 7.5)/1 mM EGTA/1 mM MgCl2/150 mM NaCl /5% glycerol/1% NP­40] containing protease inhibitors (1:100) and phosphatase inhib...
ORGANISM(S): Mus Musculus (mouse) 
2022-12-24 | PXD039092 | JPOST Repository
ProteomicsNakaya_VGLL3-FLAG_IP-MS_QEplus
Cardiac myofibroblasts retrovirally transduced with FLAG-VGLL3 or control vector were fixed with 0.1% PFA and lysed in a HEPES-RIPA buffer (20 mM HEPES-NaOH [pH 7.5]/1 mM EGTA/1 mM MgCl2/150 mM NaCl/0.25% sodium deoxycholate/0.05% SDS/1% NP-40) containing protease inhibitors (1:100), phosphatase inh...
ORGANISM(S): Mus Musculus (mouse) 
2022-12-24 | PXD039091 | JPOST Repository
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