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ProteomicsMudPIT analyses of the proteins purified by in vitro dCas9-targeted chromatin IP of telomere sequences in HeLa cells
We used a reverse chromatin immunoprecipitation (ChIP) method that takes advantage of the RNA-mediated DNA-targeting capability of Cas9 to efficiently and adaptably isolate specific genomic regions and their associated protein factors. By using purified recombinant catalytically-dead Cas9 (dCas9) co...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-10-25 | MSV000081645 | MassIVE
ProteomicsMudPIT analyses of the proteins purified by in vitro dCas9-targeted chromatin IP of the fruit fly Histone Cluster
We used a reverse chromatin immunoprecipitation (ChIP) method that takes advantage of the RNA-mediated DNA-targeting capability of Cas9 to efficiently and adaptably isolate specific genomic regions and their associated protein factors. By using purified recombinant catalytically-dead Cas9 (dCas9) co...
ORGANISM(S): Drosophila Melanogaster (ncbitaxon:7227) 
2017-10-25 | MSV000081643 | MassIVE
GenomicsTissue specific Pol II Chip-Seq in the early fly embryo
In order to idetify paused promoters in vivo, we performed tissue specific Pol II Chip-seq using mutant embryos for the dorsal gradient. We used two population of cells, either dorsal ectoderm cells (gd7 embryos) or mesodermal cells (Toll10b) embryos. ChIP-seq for Pol II in various Drosophila embryo...
ORGANISM(S): Drosophila melanogaster 
2013-05-23 | E-GEOD-36310 | biostudies-arrayexpress
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