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ProteomicsAcylated peptide enrichment utilizing lysine deacylases for lysine acylomics
Reversible acylation of lysine ε-amino groups, e.g., acetylation, succinylation, maronylation, and myristoylation, is involved in basic physiological processes such as metabolism, cell signaling and aging. In this study, we developed a novel enrichment method for acylated peptides without the use of...
ORGANISM(S): Homo Sapiens (human) 
2021-06-03 | PXD025845 | JPOST Repository
ProteomicsA Landscape of Ectodomain Shedding by Quantitative Protein Terminomics
Ectodomain shedding is a proteolytic process that regulates level and function of membrane proteins. By quantitative protein terminomics consisting of N- and C-terminal peptide enrichment, TMT-labeling and mass spectrometry, we quantified protein termini in the cultured media across 10 human cell li...
ORGANISM(S): Homo Sapiens (human) 
2021-03-05 | PXD021378 | JPOST Repository
ProteomicsPeptide probes containing a non-hydrolyzable phosphotyrosine-mimetic residue as a tool for tyrosine phosphatomics
Protein tyrosine phosphatases (PTPs) cooperate with protein tyrosine kinases to regulate intracellular tyrosine phosphorylation levels, and their dysfunction is associated with various diseases. However, due to the low abundance of PTPs in cells, comprehensive analysis requires an enrichment step pr...
ORGANISM(S): Homo Sapiens (human) 
2021-10-27 | PXD025038 | JPOST Repository
ProteomicsApplication of liquid-liquid extraction for N-terminal myristoylation proteomics
Proteins can be modified by lipids in various ways, for example by myristoylation, palmitoylation, farnesylation, and geranylgeranylation—these processes are collectively referred to as lipidation. Current chemical proteomics using alkyne lipids has enabled the identification of lipidated protein ca...
ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse) 
2023-11-09 | PXD041499 | JPOST Repository
ProteomicsSecretome analysis to elucidate metalloprotease-dependent ectodomain shedding of glycoproteins during neuronal cells differentiation
N1E-115 cells were seeded two days before sample preparation, and cultured in fresh medium containing 2% fetal bovine serum for 12 h before sample preparation. Cells were washed with serum-free medium for three times, and cultured in fresh serum-free DMEM with or without 50 μM GM6001 for 9 h. The co...
ORGANISM(S): Mus Musculus (mouse) 
2017-01-15 | PXD004699 | JPOST Repository
ProteomicsProteomics of human tau-transgenic mice reveals protein modules associated with tau pathologies
Abnormally accumulated tau protein aggregates are one of the hallmarks of dementia, including Alzheimer’s disease (AD), and are believed to play a critical role in neurodegeneration. In order to investigate proteomic alteration driven by tau aggregates, we implemented quantitative proteomics to anal...
ORGANISM(S): Mus Musculus (mouse) 
2022-07-05 | PXD031129 | JPOST Repository
ProteomicsEscherichia coli S2P family intramembrane protease RseP is engaged in the regulated sequential cleavages of FecR in the ferric citrate signaling
Escherichia coli RseP, a member of the S2P family intramembrane proteases, is involved in the activation of the σE extracytoplasmic stress response and the elimination of remnant signal peptides. It remains however unclear whether RseP has additional cellular functions. In this study, we attempted t...
ORGANISM(S): Escherichia Coli 
2021-04-14 | PXD023797 | JPOST Repository
ProteomicsTMPRSS2-induced Golgi disruption restricts the incorporation of virus envelope glycoproteins into virions
Understanding the relationship between viral proteins and host factors is essential for developing strategies to control virus infections. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infects host cells using angiotensin converting enzyme 2 (ACE2) and transmembrane protease, serine 2...
ORGANISM(S): Homo Sapiens (human) 
2026-04-15 | PXD065094 | JPOST Repository
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