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In this project, we isolate U1 snRNA associated proteins in Arabidopsis thaliana. We used an antisense oligonucleotide specific for the U1 snRNA and analyzed associated proteins by mass spectrometry. As a control, the same experiments were performed with U2 snRNA- and lacZ-specifc antisense oligonuc...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2023-12-04 | PXD045484 | Pride
U1 small nuclear (sn)RNA, required for splicing of pre-mRNA, is encoded by genes on chromosome 1p36. Imperfect copies of these ‘true’ (t)U1 snRNA genes, located on chromosome 1q12-21, were thought to be pseudogenes. However, many of these ‘variant’ (v)U1 snRNA genes produce fully-processed transcrip...
ORGANISM(S): Homo sapiens 
Exogenous expression analysis of U1 snRNA mutation
Transcriptome analysis of U1 snRNA overexpression in human neurons
Effect of in vitro-transcribed U1 snRNA on gene expression
The experiment was carried out to examine the effect of Srrt knockdown on the U1 snRNP/pre-mRNA interaction pattern. A2Lox mouse embryonic stem cells were transfected with either an Srrt-specific or a non-targeting siRNA. 48 hours post transfection the cells were cross-linked using 2% formaldehyde a...
ORGANISM(S): Mus musculus 
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