Characterization of RNA processing events dependent on U2AF-related proteins PUF60 and RBM39. PUF60 (poly-U-binding factor 60 kDa, also known as FIR, Hfp or Ro-bp1) is a splicing factor homologous to the 65 kD subunit of the auxiliary factor of U2 small nuclear ribonucleoprotein (U2AF65). PUF60 has...
The aim of the experiment is to reveal splicing isoforms induced by transient expression of Fritillaria borealis U2AF in HEK293T cells. Cells were transfected with different combinations of U2AF and U2AF2 subunits paralogues. A cycloheximide treatment was applied to inhibit nonsense-mediated decay a...
The U2AF heterodimer has been well studied for its role in defining functional 3M-bM-^@M-^Y splice sites in pre-mRNA splicing, but many fundamental questions still remain unaddressed regarding the function of U2AF in mammalian genomes. Through genome-wide analysis of U2AF-RNA interactions, we report...
