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ProteomicsQuantitative nascent proteome profiling by pulse labeling of O-propargyl-puromycin and stable isotope labeled amino acids
Recently, a clickable puromycin analog, O-propargyl-puromycin (OPP), was developed and applied to label C-termini of nascent polypeptide chains (NPCs), followed by a proteomic analysis of NPCs that can be affinity purified via click reaction. Despite its advantage, the affinity purification of NPCs ...
ORGANISM(S): Homo Sapiens (human) 
2020-09-17 | PXD019459 | JPOST Repository
ProteomicsProteome-wide analysis of elongating nascent polypeptide chains – IP input
Cellular global translation is often measured using ribosome profiling or quantitative mass spectrometry, but these methods do not provide direct information at the level of elongating nascent polypeptide chains (NPCs) and associated co-translational events. We combine quantitative proteomics, pulse...
ORGANISM(S): Homo Sapiens (human) 
2022-02-09 | PXD024079 | JPOST Repository
ProteomicsProteome-wide analysis of elongating nascent polypeptide chains – CX4945 treatment
Cellular global translation is often measured using ribosome profiling or quantitative mass spectrometry, but these methods do not provide direct information at the level of elongating nascent polypeptide chains (NPCs) and associated co-translational events. We combine quantitative proteomics, pulse...
ORGANISM(S): Homo Sapiens (human) 
2022-02-09 | PXD024080 | JPOST Repository
ProteomicsProteome-wide analysis of elongating nascent polypeptide chains - mouse primary neuron DIV5 vs DIV14
Cellular global translation is often measured using ribosome profiling or quantitative mass spectrometry, but these methods do not provide direct information at the level of elongating nascent polypeptide chains (NPCs) and associated co-translational events. We combine quantitative proteomics, pulse...
ORGANISM(S): Mus Musculus (mouse) 
2022-05-28 | PXD026346 | JPOST Repository
ProteomicsProteome-wide analysis of elongating nascent polypeptide chains – peptide pulldown
Cellular global translation is often measured using ribosome profiling or quantitative mass spectrometry, but these methods do not provide direct information at the level of elongating nascent polypeptide chains (NPCs) and associated co-translational events. We combine quantitative proteomics, pulse...
ORGANISM(S): Homo Sapiens (human) 
2022-05-28 | PXD026349 | JPOST Repository
ProteomicsNovel roles of small extracellular vesicles in regulating the quiescence and proliferation of neural stem cells
We analyzed protein cargos of NSC sEVs from proliferating, resting and reactivating status by proteomics approaches. Our results revealed specific functional clusters of pathways in gene ontology annotations of differentially expressed proteins in three sources of exosomes. Furthermore, we showed th...
ORGANISM(S): Mus Musculus (mouse) 
2021-10-27 | PXD027651 | JPOST Repository
ProteomicsProteome-wide analysis of elongating nascent polypeptide chains – LPS stimulation to RAW264.7 macrophages
Cellular global translation is often measured using ribosome profiling or quantitative mass spectrometry, but these methods do not provide direct information at the level of elongating nascent polypeptide chains (NPCs) and associated co-translational events. We combine quantitative proteomics, pulse...
ORGANISM(S): Mus Musculus (mouse) 
2023-02-07 | PXD031475 | JPOST Repository
ProteomicsProteome-wide analysis of elongating nascent polypeptide chains - pSNAP evaluation
Cellular global translation is often measured using ribosome profiling or quantitative mass spectrometry, but these methods do not provide direct information at the level of elongating nascent polypeptide chains (NPCs) and associated co-translational events. We combine quantitative proteomics, pulse...
ORGANISM(S): Homo Sapiens (human) 
2023-02-07 | PXD024078 | JPOST Repository
ProteomicsProteome-wide analysis of elongating nascent polypeptide chains - mouse primary neuron
Cellular global translation is often measured using ribosome profiling or quantitative mass spectrometry, but these methods do not provide direct information at the level of elongating nascent polypeptide chains (NPCs) and associated co-translational events. We combine quantitative proteomics, pulse...
ORGANISM(S): Mus Musculus (mouse) 
2023-02-07 | PXD024104 | JPOST Repository
ProteomicsProteome-wide analysis of elongating nascent polypeptide chains – Nt-acetylation analysis
Cellular global translation is often measured using ribosome profiling or quantitative mass spectrometry, but these methods do not provide direct information at the level of elongating nascent polypeptide chains (NPCs) and associated co-translational events. We combine quantitative proteomics, pulse...
ORGANISM(S): Homo Sapiens (human) 
2022-02-09 | PXD024081 | JPOST Repository
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