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MetabolomicsMetabolic Footprinting of a Clear Cell Renal Cell Carcinoma in vitro Model for Human Kidney Cancer Detection
A protocol for harvesting and extracting extracellular metabolites from an in vitro model of human renal cell lines was developed to profile the exometabolome by means of a discovery-based metabolomics approach using ultraperformance liquid chromatography coupled to quadrupole-time-of-flight mass sp...
2018-11-14 | MTBLS737 | MetaboLights
MetabolomicsFlow cytometry has a significant impact on the cellular metabolome (CE-MS assay)
The characterization of specialized cell subpopulations in a heterogeneous tissue is essential for understanding organ function in health and disease. A popular method of cell isolation is fluorescence-activated cell sorting (FACS) based on probes that bind surface or intracellular markers. In this ...
2018-11-06 | MTBLS633 | MetaboLights
ProteomicsIntegrated amino acid profiling and 4D-DIA proteomics reveal protein quality divergence and metabolic adaptation in Cordyceps species
To explore the differences in protein quality among classic medicinal entomopatho-genic fungi and to evaluate their metabolic adaptability, we analyzed the amino acid composition and proteomic characteristics of Cordyceps sinensis (CS), Cordyceps militaris (CM) and Cordyceps cicadae (CC). Quantitati...
ORGANISM(S): Ophiocordyceps Sinensis Cordyceps Militaris Cordyceps Cicadae 
PXD063259 |
TranscriptomicsHDACs and Phosphorylated Pol II CTD recruit Spt6 for cotranscriptional histone reassembly
Spt6 is a multifunctional histone chaperone involved in the maintenance of chromatin structure during elongation by RNA polymerase II (Pol II). Spt6 has a tandem SH2 (tSH2) domain within its C-terminus that recognizes Pol II CTD peptides phosphorylated on Ser2, Ser5 or Try1 in vitro. Deleting the tS...
ORGANISM(S): Saccharomyces cerevisiae 
2014-09-25 | E-GEOD-61713 | biostudies-arrayexpress
TranscriptomicsGenes involved in synthesis of lipoteichoic acids are induced when Bifidobacterium animalis subsp. lactis BB12 is incubated with Human Enterocyte-Like Caco-2 Cells
Bifidobacterium animalis subsp. lactis BB12 is a widely used probiotic bacterium. However the molecular mechanism by which this bacterium confers positive health effects to the host is largely unknown. The importance of lipoteichoic acids (LTA) in the cross-talk between the bacteria and human entero...
ORGANISM(S): Bifidobacterium animalis subsp. lactis BB-12 
2012-07-31 | E-GEOD-21588 | biostudies-arrayexpress
UnknownRisk prediction in stable cardiovascular disease using a high-sensitivity cardiac troponin T single biomarker strategy compared to the ESC-SCORE.
Not available
S-EPMC5922562 | biostudies-literature
MetabolomicsEnzyme engineering for optimizing biosynthesis of 2,4-dihydroxybutyric acid via the synthetic threose-dependent glycolaldehyde assimilation (STEGA) pathway
Ethylene glycol is a potential feedstock for next-generation biorefineries, as it can be derived from both plastic waste and carbon dioxide. The synthetic D-threose-dependent glycolaldehyde assimilation (STEGA) pathway, which is orthogonal to central metabolism of Escherichia coli, was recently cons...
2026-07-15 | MTBLS15050 | MetaboLights
TranscriptomicsEvolutionarily conserved C-terminal region of TAF9 is critical for SAGA and TFIID recruitment to promoters and transcriptional activation
TFIID and SAGA complexes play a critical role in RNA Polymerase II dependent activated transcription. Although the two regulatory complexes are recruited to promoters by activation domain-interactions, the contribution of the different subunits or the different domains of the individual subunits is ...
ORGANISM(S): Saccharomyces cerevisiae 
2014-02-21 | E-GEOD-44544 | biostudies-arrayexpress
MetabolomicsEcophysiological Study of Paraburkholderia sp. Strain 1N under Soil Solution Conditions: Dynamic Substrate Preferences and Characterization of Carbon Use Efficiency

We used time-resolved metabolic footprinting, an important technical approach used to monitor changes in extracellular compound concentrations during microbial growth, to study the order of substrate utilization (i.e., substrate preferences) and kinetics of a fast-growing soil isolate, Parabu...

2020-09-28 | MTBLS1692 | MetaboLights
GenomicsGenome-wide cooperation by HAT Gcn5, remodeler SWI/SNF, and chaperone Ydj1 in promoter nucleosome eviction and transcriptional activation
Chaperones, nucleosome remodeling complexes and histone acetyltransferases have been implicated in nucleosome disassembly at promoters of particular yeast genes, but whether these co-factors function ubiquitously, and the impact of nucleosome eviction on transcription genome-wide, are poorly underst...
ORGANISM(S): Saccharomyces cerevisiae 
2015-11-09 | E-GEOD-74787 | biostudies-arrayexpress
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