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Proteins that are vital to cell survival can become unfolded during heat stress, leading to the formation of toxic aggregates. Molecular chaperones, proteases and autophagic pathways are required to protect cells from the accumulation of protein aggregates. Small Heat Shock Proteins (sHSPs) form a f...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2019-05-14 | PXD011483 | Pride
The Organomercury-resin-assisted capture (MRC) of S-nitrosoproteins was done with modifications as described in (Doulias and Gould, 2018). Entire inflorescences pre-anthesis (corresponding to FD stages up to 12/13) of soil grown WT and hot5-2 plants were harvested, shock frozen in lN2 and ground to ...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2024-02-21 | MSV000094148 | MassIVE
Leaves from 4 to 6 week old soil grown WT Col 0 and gsnor1/hot5_2 plants were used for protein extraction. For that, 200mg of plant material was resuspended in extraction buffer (25mM HEPES pH 7.7, 1mM EDTA, 2.5% [w/v] SDS, protease inhibitor cocktail (Pierce A32955)). Following TCA precipitation, p...
ORGANISM(S): Arabidopsis 
2021-08-24 | MSV000088017 | MassIVE
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