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L. lactis was grown in steady-state cultures at specific growth rate 0.2 h-1. The concentration of L-threonine in the growth environment was smoothly increased from 1.24mM to 4mM and proteome measurements were carried out to detect proteins involved in threonine metabolism.
ORGANISM(S): Lactococcus lactis 
2015-03-19 | PXD001602 | Pride
Specific growth rate dependent gene expression changes of Escherichia coli K12 MG1655 were determined by microarray and real time PCR analyses. The bacteria were cultivated on glucose limited minimal medium using the accelerostat method (A-stat), where starting from steady state conditions in a chem...
ORGANISM(S): Escherichia coli 
Cells usually respond to changing growth conditions with a change in the specific growth rate (μ) and adjustment of their proteome to adapt and maintain metabolic efficiency. Description of the principles behind proteome resource allocation is thus important for understanding metabolic regulation in...
ORGANISM(S): Escherichia coli 
2015-02-27 | PXD001594 | Pride
E. coli BW25113 growth in a nutrient-rich medium supplemented with 20 proteinogenic amino acid medium was studied and compared to growth in minimal medium. Proteome data from these experiments revealed significant differences in amino acid synthesis and transport proteins that were reduced in the nu...
ORGANISM(S): Escherichia coli 
2020-02-18 | PXD011726 | Pride
Dihydroxyacetone (DHA) is an attractive molecule produced in a wide range of industries . DHA is found among all the kingdoms as an intermediate of various metabolic pathways and can be used as a carbon source by many organisms. The bacterium Escherichia coli is able to grow on DHA as the sole carbo...
ORGANISM(S): Escherichia coli K-12 
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