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GenomicsG&T-seq: parallel sequencing of the genomes and transcriptomes of single cells
The ability to simultaneously sequence the genome and transcriptome of the same single cell offers a powerful means to dissect functional genetic heterogeneity at the cellular level. Here we describe G&T-seq, a method for separating and sequencing genomic DNA and full-length mRNA from single cells. ...
EGAS00001001204 | EGA
TranscriptomicsSingle-cell copy number varation detection
Detection of genomic rearrangements from a single cell instead of a population of cells is an emerging research technique with important applications in the study of human fertility, constitutional chromosomal disorders, and tumor progression. Here, we develop a method to improve the detection of si...
ORGANISM(S): Homo sapiens 
2011-08-23 | E-GEOD-27892 | biostudies-arrayexpress
GenomicsEGAS00001001204-sc-20150415 - samples
Development of a method for separation and parallel sequencing of the genomes and transcriptomes of single cells.
EGAD00001001332 | EGA
TranscriptomicsSNP array analysis of single cell genomes
This study (McConnell, et al. Science 2012) used both SNP array and sequencing data to examine copy number variation in neuronal genomes. Encolsed here are the SNP Array data from the 42 fibroblasts, 19 human induced pluripotent stem cell (hiPSC)-derived neural progenitor cells (NPCs), and 40 hiPSC-...
ORGANISM(S): Homo sapiens 
2013-12-04 | E-GEOD-51538 | biostudies-arrayexpress
GenomicsParallel single-cell bisulfite- and RNA-sequencing link transcriptional and epigenetic heterogeneity [Bisulfite-Seq]
We report scM&T-seq, a method for parallel single-cell genome-wide methylome and transcriptome sequencing, allowing discovery of associations between transcriptional and epigenetic variation. Profiling of 61 mouse embryonic stem cells confirmed known links between DNA methylation and transcription. ...
ORGANISM(S): Mus musculus 
2015-12-24 | E-GEOD-68642 | biostudies-arrayexpress
TranscriptomicsParallel single-cell bisulfite- and RNA-sequencing link transcriptional and epigenetic heterogeneity [RNA-seq]
We report scM&T-seq, a method for parallel single-cell genome-wide methylome and transcriptome sequencing, allowing discovery of associations between transcriptional and epigenetic variation. Profiling of 61 mouse embryonic stem cells confirmed known links between DNA methylation and transcription. ...
ORGANISM(S): Mus musculus 
2015-12-24 | E-GEOD-74534 | biostudies-arrayexpress
TranscriptomicsNon-allelic homologous recombination between retrotransposable elements is a major driver of de novo unbalanced translocations.
Large scale analysis of balanced chromosomal translocation breakpoints has shown nonhomologous end joining and microhomology-mediated repair to be the main drivers of interchromosomal structural aberrations. Breakpoint sequences of de novo unbalanced translocations have not yet been investigated sys...
ORGANISM(S): Homo sapiens 
2012-07-12 | E-GEOD-39303 | biostudies-arrayexpress
TranscriptomicsSingle-cell copy number varation detection: blastomeres
Detection of genomic rearrangements from a single cell instead of a population of cells is an emerging research technique with important applications in the study of human fertility, constitutional chromosomal disorders, and tumor progression. Here, we develop a method to improve the detection of si...
ORGANISM(S): Homo sapiens 
2011-08-09 | E-GEOD-31219 | biostudies-arrayexpress
TranscriptomicsGenome-wide haplotyping of human single cells
A method to infer genome-wide haplotypes from the analysis of one or two single (human) cells has tremendous applicative value. It would revolutionize not only preimplantation genetic diagnosis of in vitro fertilized human embryos in the clinic, but also animal breeding programs by enabling genome-w...
ORGANISM(S): Homo sapiens 
2012-10-01 | E-GEOD-17318 | biostudies-arrayexpress
Genomicsena-DATASET-SC-21-07-2014-10:55:39:527-89 - samples
NSCLC targeted.
EGAD00001000889 | EGA
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