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ProteomicsA universal method for the rapid isolation of all known classes of functional small RNAs
Diverse classes of regulatory small (s)RNAs operate via ARGONAUTE-family proteins within RNA-induced-silencing-complexes (RISCs). Based the conserved biochemical properties intrinsic to all ARGONAUTEs, we have developed a universal, 15-min benchtop extraction procedure allowing simultaneous purifica...
ORGANISM(S): Caenorhabditis elegans 
2021-09-08 | PXD015395 | Pride
Argonaute Mrna binding proeins Small rna 
TranscriptomicsDeep-sequencing influences the results obtained in small-RNA sequencing
This study presents a comparison of small RNA sequencing libraries generated from the same cell lines but using different sequencing platforms and protocols. The samples were analyzed and compared at the level of miRNAs expression and as a population of small RNAs derived from repetitive elements. D...
ORGANISM(S): Mus musculus 
2012-03-01 | E-GEOD-35368 | biostudies-arrayexpress
TranscriptomicsAntiviral RNA interference in mammalian cells
Plants and invertebrates protect themselves from viruses through RNA interference (RNAi), yet it remains unknown whether this defense mechanism exists in mammals. Antiviral RNAi involves the processing of viral long double-stranded (ds) RNA molecules into small interfering RNAs (siRNAs) by the ribon...
ORGANISM(S): Mus musculus 
2013-10-10 | E-GEOD-43153 | biostudies-arrayexpress
Transcriptomicschip in silencing suppressor plants or in mirna plants-Transcriptional and post-transcriptional changes in Arabidopsis plants that constitutively express suppressors of RNA silencing
gen107_ptgs - chip in silencing suppressor plants or in mirna plants - What are the genes that are differentially regulated in various tissues derived from silencing suppressor plants or miRNA mutants? - How are the histone modifications and the chromatin structure in miRNA mutants or in silencing s...
ORGANISM(S): Arabidopsis thaliana 
2011-08-22 | E-GEOD-24692 | biostudies-arrayexpress
TranscriptomicsRestriction of LINE1 activity by RNAi correlates with mouse ES cell differentiation
Long interspersed elements 1 (LINE-1 or L1) are retrotransposons that dominate the mouse genomic landscape, and are expressed in Embryonic Stem Cells (ESCs), germ cells, and during early development. Based on clear precedents in plants and fission yeast, we investigated in this study a role for RNAi...
ORGANISM(S): Mus musculus 
2013-12-11 | E-GEOD-43110 | biostudies-arrayexpress
Transcriptomicsgene profiling in silencing suppressor plants or in mirna mutants-Transcriptional and post-transcriptional changes in Arabidopsis plants that constitutively express suppressors of RNA silencing
gen107_ptgs - gene profiling in silencing suppressor plants or in mirna mutants - What are the genes that are differentially regulated in various tissues derived from silencing suppressor plants or miRNA mutants? - HcPro, P15, P19, CHS-RNAi (control), were grown on MS solid medium, and tissues were ...
ORGANISM(S): Arabidopsis thaliana 
2011-08-22 | E-GEOD-24693 | biostudies-arrayexpress
TranscriptomicsHigh-throughput pyrosequencing of endogenous small RNAs from Arabidopsis thaliana wild-type and ddm1 mutant.
DNA methylation is essential for silencing transposable elements and some genes in higher eukaryotes, implying that this modification must be tightly controlled. However, accidental changes in DNA methylation can be transmitted through mitosis, as in cancer, or meiosis, leading to epiallelic variati...
ORGANISM(S): Arabidopsis thaliana 
2009-02-02 | E-GEOD-13419 | biostudies-arrayexpress
Transcriptomicscomparison of transcriptomes in mad mutants-Transcriptome analysis of miRNA action deficient mutants
rs10-04_mad - comparison of transcriptomes in mad mutants - What gene sets are differentially expressed in mad mutants? - Seeds of GFP171.1 (parental line), mad1, mad2, mad3, mad6 and dcl1-12 were sterilized and germinated on Murashige/Skoog medium with 0.9% agar. Plates were stratified at 4C in the...
ORGANISM(S): Arabidopsis thaliana 
2011-09-14 | E-GEOD-30477 | biostudies-arrayexpress
TranscriptomicsComparison of IND induced vs. mock treated seedlings: Transcriptome profiling upon induction of the transcription factor IND
rs10-03_ind - comparison of ind induced vs mock treated seedlings - Identification of miRNAs regulated by IND - Transgenic seeds containing an IND transgene under the control of a dexamethasone inducible promoter were germinated and grown in liquid Murashige/Skoog medium under a 16h light/8h darknes...
ORGANISM(S): Arabidopsis thaliana 
2011-05-12 | E-GEOD-28898 | biostudies-arrayexpress
TranscriptomicsSmall RNA profiling in an Arabidopsis transgenic line overexpressing AtmiR472
Virus resistance relies in some plant-viral interactions on the RNA-DEPENDANT RNA POLYMERASE 6 (RDR6), a major actor of RNA silencing that acts at the post-transcriptional level. Here, we demonstrate that RDR6 also plays a role in basal defense and race-specific resistance. RDR6 and the microRNA miR...
ORGANISM(S): Arabidopsis thaliana 
2013-12-19 | E-GEOD-53484 | biostudies-arrayexpress
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