Spermiogenesis is a tightly regulated process to produce mature sperm cells. The ubiquitin-proteasome system (UPS) plays a crucial role in controlling protein half-life and is essential for spermiogenesis. In this study, we demonstrate that UBL7, a testis-enriched UBL-UBA protein, is indispensable f...
Protein acetylation, one of many types of post-translational modifications (PTMs), is involved in a variety of biological and cellular processes. In the present study, we applied both CsCl density gradient (CDG) centrifugation-based protein fractionation and a dimethyl-labelling-based 4C quantitativ...
An in planta chemical cross-linking-based quantitative interactomics (IPQCX-MS) workflow has been developed to investigate in vivo protein-protein interactions and alteration in protein structures in a model organism, Arabidopsis thaliana. A chemical cross-linker, azide-tag-modified disuccinimidyl p...
To investigate the important function of membrane protein TMEM225 in sperm, we constructed Tmem225 knockout mice. Sperm from knockout mice and control mice were isolated for proteomic analysis.
The ddaPASEF and diaPASEF data were acquired from over 60 types of human specimens. Based on these, the consensus spectral libraries were generated and used in subsequent DIA analysis to demonstrate a quantitative human proteome map and discover potential cancer biomarkers.
