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De novo synthesized protein DARPin F10 can target HDAC6 ZnF domain, and inhibits Influenza A virus and ZIKA virus infection. Our data shows it interferes with the virus uncoating step. To exclude the possibility that F10 upregulates interferon pathways, we checked the transcription of the cell
ORGANISM(S): Homo sapiens 
THP-1 cells were infected by IAV virus strain X31 with a MOI=10, and cells were collected at 3 timepoints:0 hr, 24 hr and 48 hr post-infection. Each timepoint has 3 biological replicates.
ORGANISM(S): Homo sapiens 
Designed protein UBD10 can bind to Ub, and here we used AP-MS to check its major interacting proteins.celline is A549, and lysed by CoIP buffer and incubated with GST-Trap beads (either prebound with GST-UBD10 and GST)
ORGANISM(S): Homo sapiens (Human) 
2026-08-13 | PXD056512 | Pride
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