Protein extraction and proteolytic digestion were performed using a Filter-Assisted Sample Preparation (FASP) protocol. In case of phosphopeptide enrichment immobilized Fe(III) affinity chromatography (Fe-IMAC) was performed. The peptides were fractionated using an HPLC system fitted with an SCX col...
Data from ProteomeXchange, PXD ID: PXD001200. File: 1300809_AAS2set_10uM.mzml. Data publsihed as part of J Proteome Res. 2014 Sep 30 . From the Abstract: {{i}} ATP-binding proteins, including protein kinases, play essential roles in many biological and pathological processes and thus these proteins...
Data from ProteomeXchange, PXD ID: PXD000851. File: iTRAQ3.msf.mgf. Published as part of Mol Cell Proteomics. 2014 Mar 31 . From the Abstract: {{i}} We here performed a quantitative proteomic analysis of membrane fractions from colorectal cancer tissue to discover biomarker candidates, and then ext...