Islet lysates were adjusted to 5 percent SDS in TEAB, pH 8.5, reduced and alkylated and digested with modified trypsin (Worthington) using an S-trap mini device (Protifi). Peptides were labeled with TMT16 reagents (lot ZH350094). Phosphopeptide enrichment used GL Sciences p10 TiO tips. LC-MS/MS used...
Metabolomics data from cell culture; Treated with wild-type MERS-CoV, MERS-CoV mutants and mockulum; 5 biological replicates; time points 0, 12, 24, 36 & 48 h
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) Middle East Respiratory Syndrome-related Coronavirus (ncbitaxon:1335626)
Lipidomics data from Calu-3 cell culture; Treated with wild-type and mutant H7N9 and mockulum; Time points 0, 3, 7, 12, 18 & 24 h; 5 biological replicates
Lipidomics data from Calu-3 cell culture; Treated with wild-type and mutant H5N1 and mockulum; Time points 0, 3, 7, 12, 18 & 24 h; 5 biological replicates