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ProteomicsPatch-Clamp Proteomics of Single Neurons in Tissue Using Electrophysiology and Subcellular Capillary Electrophoresis Mass Spectrometry
In this project, we enabled mass spectrometry based proteomics characterization of single neurons in sections of the mouse brain. We accessed cells in a whole-cell patch clamp configuration and aspirated a portion of the neuronal soma into the patch clamp probe. The collected aspirate was expelled ...
ORGANISM(S): Mus musculus (Mouse) 
2022-02-18 | PXD028040 | Pride
Electrophysiology Mass spectrometry Nervous system Bottom-up proteomics Capillary electrophoresis Whole-cell patch-clamp Mouse 
UnknownAutomated whole-cell patch-clamp electrophysiology of neurons in vivo.
Not available
S-EPMC3427788 | biostudies-literature
UnknownRobotic Automation of In Vivo Two-Photon Targeted Whole-Cell Patch-Clamp Electrophysiology.
Not available
S-EPMC5584670 | biostudies-literature
TranscriptomicsSingle-cell RNA sequencing of aspirates from cortical neurons after patch clamp recording
We obtained full transcriptome data from single cortical neurons after whole-cell patch-clamp recording (termed “Patch-seq”). By applying “Patch-seq” to cortical neurons, we reveal a close link between biophysical membrane properties and genes coding for neurotransmitter receptors and channels, incl...
ORGANISM(S): Mus musculus 
2015-12-21 | GSE70844 | GEO
UnknownComputer modeling of whole-cell voltage-clamp analyses to delineate guidelines for good practice of manual and automated patch-clamp.
Not available
S-EPMC7870888 | biostudies-literature
UnknownDirect whole-cell patch-clamp recordings from small boutons in rodent primary neocortical neuron cultures.
Not available
S-EPMC10026040 | biostudies-literature
UnknownDendritic patch-clamp recording.
Not available
S-EPMC7616975 | biostudies-literature
UnknownMethod for Rapid Enzymatic Cleaning for Reuse of Patch Clamp Pipettes: Increasing Throughput by Eliminating Manual Pipette Replacement between Patch Clamp Attempts.
Not available
S-EPMC8329470 | biostudies-literature
UnknownDetergent induction of HEK 293A cell membrane permeability measured under quiescent and superfusion conditions using whole cell patch clamp.
Not available
S-EPMC3983356 | biostudies-literature
UnknownA Comparative Study on the Lysosomal Cation Channel TMEM175 Using Automated Whole-Cell Patch-Clamp, Lysosomal Patch-Clamp, and Solid Supported Membrane-Based Electrophysiology: Functional Characterization and High-Throughput Screening Assay Development.
Not available
S-EPMC10454728 | biostudies-literature
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