Sort   by:  
 Page size 
TranscriptomicsThe effect of TGF-beta in Apc-mutated mouse intestinal organoids
Intestinal crypts isolated from Apcflox/flox; villin-CreERT mice were treated with Tamoxifen to induce the deletion of Apc. Tamoxifen-treated organoids were selected in the absence of Wnt agonists and then treated with TGF-beta. Total RNA obtained from Tamoxifen-treated, Apc-deleted intestinal organ...
ORGANISM(S): Mus musculus 
2014-04-18 | E-GEOD-35093 | biostudies-arrayexpress
TranscriptomicsApc, Kras, and TGFbeta in intestinal organoids
This SuperSeries is composed of the SubSeries listed below. Refer to individual Series
ORGANISM(S): Mus musculus 
2014-04-18 | E-GEOD-35094 | biostudies-arrayexpress
TranscriptomicsGene expression changes in oncogenic Kras-containing, Apc-mutated mouse intestinal organoids in the presence or absence of TGF-beta
We isolated and selected intestinal adenoma organoids from Apc1638N/+ and Apc1638N/+; Kras mice. After the selection procedure, we maintained the cultures with or without TGF-beta for 18h. RNA was isolated to determine the effect of oncogenic Kras on the gene expression changes. Total RNA obtained f...
ORGANISM(S): Mus musculus 
2014-04-18 | E-GEOD-35092 | biostudies-arrayexpress
TranscriptomicsGene expression changes in Apc-mutant mouse intestinal organoids with and without deleting the Prox1 transcription factor
We isolated and selected intestinal adenoma organoids from villin-CreER; Apcflox/flox and villin-CreER; Apcflox/flox; Prox1flox/flox mice and added tamoxifen to induce the deletion of the Apc and Prox1 genes in the intestinal epitheliul ex vivo. Microarray experiments were carried out 7 days after ...
ORGANISM(S): Mus musculus 
2014-08-15 | E-GEOD-47568 | biostudies-arrayexpress
TranscriptomicsGene expression changes in Apc-mutant mouse intestinal organoids 7 and 20 days after the induction of the Apc deletion
We isolated and selected intestinal adenoma organoids from Lgr5-EGFP-IRES-CreER; Apcflox/flox mice and added tamoxifen to induce the deletion of the Apc gene in the intestinal stem cells. Gene expressions on day7 and day20 after the addition of tamoxifen were compared, representing two stages with d...
ORGANISM(S): Mus musculus 
2015-12-31 | E-GEOD-47569 | biostudies-arrayexpress
MetabolomicsDirect on-swab metabolic profiling of vaginal microbiome:host interactions during pregnancy and preterm birth

The pregnancy vaginal microbiome contributes to risk of preterm birth, the primary cause of death in children under 5 years of age. Here we describe direct on-swab metabolic profiling by Desorption Electrospray Ionization Mass Spectrometry (DESI-MS) for sample preparation-free characterisation of...

2021-09-23 | MTBLS717 | MetaboLights
Sort   by:  
 Page size