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TranscriptomicsEgr2-dependent expression in T cell anergy
T cell anergy is one of the mechanisms contributing to peripheral tolerance, particularly in the context of progressively growing tumors and in tolerogenic treatments promoting allograft acceptance. We recently reported that early growth response gene 2 (Egr2) is a critical transcription factor for...
ORGANISM(S): Mus musculus 
2013-04-21 | E-GEOD-46242 | biostudies-arrayexpress
ProteomicsIntegrative proteomics and phosphoproteomics profiling and systems biology analysis reveal dynamic signaling networks and mTORC1-dependent mitochondrial function in T cell activation
The underlying mechanisms by which naïve T cells exit from quiescence after antigen stimulation remain elusive. Using multiplex isobaric labeling proteomics technology, we report unbiased, temporal profiling of whole proteome and phosphoproteome during the activation in the wild type and Rptor-/- T ...
ORGANISM(S): Mus musculus (Mouse) 
2017-03-29 | PXD005492 | Pride
System biology T cell activation Mtorc1 Mass spectrometry Rptor Proteomic Phosphoproteomics Mitochondrial function 
TranscriptomicsSmall RNA profiling of wildtype and Eri1-deficient mouse T cells
Natural killer (NK) cells play a critical role in early host defense to infected and transformed cells. Here we show that mice deficient in Eri1, a conserved 3M-bM-^@M-^Y-to-5M-bM-^@M-^Y exoribonuclease that represses RNA interference, have a cell-intrinsic defect in NK cell development and maturati...
ORGANISM(S): Mus musculus 
2012-07-11 | E-GEOD-31920 | biostudies-arrayexpress
TranscriptomicsRegulation of gene expression by RORgamma in Th17 cells
We observed significant growth inhibition of melanoma tumor in ROR?-/- mice, suggesting an important role for the T helper 17 cell (TH17) pathway in tumor immunity. Though RORgamma t (ROR?t) plays a critical role in the development of IL-17-secreting Th17 cells, the role of other genes remained an o...
ORGANISM(S): Mus musculus 
2013-04-17 | E-GEOD-38704 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human embryonic kidney cells HEK293 overexpressing the transcription factors HNF4a2 or mutant forms HNF4a2 C106R and HNF4a2 R154X to identify HNF4a regulated genes in kidney cells being several of these genes deregulated in renal cell carcinoma
Analysis of gene expression in human embryonic kidney cells HEK293 overexpressing the transcription factors HNF4a2 or mutant forms HNF4a2 C106R and HNF4a2 R154X, which were introduced by FRT/FLP recombination. The analysis was performed 24 hours following 1sg/ml tetracycline treatment to induce the ...
ORGANISM(S): Homo sapiens 
2007-10-30 | E-GEOD-2700 | biostudies-arrayexpress
GenomicsGenome-wide maps of EGR2 transcription factor binding sites in T cell anergy
We report the application of sequencing technology for mapping the EGR2 transcriptional program in T cell anergy Examination of Egr2 genomic binding sites in T cells following overnight treatment with immobilized anti-CD3 mAb (1ug/ml)
ORGANISM(S): Mus musculus 
2013-04-21 | E-GEOD-46241 | biostudies-arrayexpress
TranscriptomicsGeneration of NPM1-ALK translocation in human T lymphocytes faithfully recapitulates the diversity of ALK+ ALCL tumor phenotype
To recapitulate the t(2;5)(p23;q35) chromosomal translocation, activated T lymphocytes from healthy donors PBMC were transfected with the RNP complex composed of the protein Cas9 with gRNA NPM1 (targeting NPM1 gene) and gRNA ALK (targeting ALK gene). Control cells were wild-type CD4+ activated lymp...
ORGANISM(S): Homo sapiens 
2022-02-09 | E-MTAB-10924 | biostudies-arrayexpress
TranscriptomicsNext Generation Sequencing Facilitates Quantitative Analysis of Wild Type, DDX5 knockout, RORc knockout, RmrpG262T knockin T helper 17 cell Transcriptomes [RNA-seq]
Purpose: The goals of this study are to compare in vitro polarized T helper 17 cell transcriptome profiling (RNA-seq) in different genetic background. Methods: Th17 mRNA profiles of 96hrs in vitro cultured T helper 17 cells from wild-type and mutant mice were generated by deep sequencing, using Ill...
ORGANISM(S): Mus musculus 
2015-12-21 | E-GEOD-70108 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of E. coli K-12 yliH and yceP biofilm expression relative to wild type
Expression in biofilm cells of mutants yceP and yliH relative to biofilm cells of E. coli K-12 wild type strain Experiment Overall Design: All strain cultured in LB glu, biofilm isolated from glass wool after culturing for 24 hrs.
ORGANISM(S): Escherichia coli 
2008-06-12 | E-GEOD-3937 | biostudies-arrayexpress
TranscriptomicsSingle cell RNA sequencing of murine gamma delta T cells from wild type and beta2 integrin-deficient (CD18 KO) lungs
The beta2 integrin subunit (CD18) is thought to regulate gamma delta T cells in vivo. The objective of this study was to determine the gene expression changes mediated by loss of CD18 in lung gamma delta T cells. Single cell RNA sequencing was performed on gamma delta T cells (CD3+ gamma delta TCR+)...
ORGANISM(S): Mus musculus 
2020-08-14 | E-MTAB-8732 | biostudies-arrayexpress
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