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Autofluorescent erythroid cells (defined as TER-119+) were compared to Nkx2-5-GFP+ heterozygous cells at E9.5.
ORGANISM(S): Mus musculus 
Nkx2-5-GFP+ cells were collected by FACS from whole embryos (E8.0-8.5, 5-12 ps) and dissected heart tubes (E9.0-9.5, 15-21 ps). Because limited numbers of cells were obtained from individual embryos (~300 cells at E8, ~1500 cells at E9.5) mRNA was amplified twice with T7 RNA polymerase and fluoresce...
ORGANISM(S): Mus musculus 
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