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TranscriptomicsComparison of wild-type and cell death mutant of barley plants containing Mla6 powdery mildew resistance gene
Time-course expression profiles of Bgh challenged barley cultivar C.I. 16151 (harboring the Mla6 powdery mildew resistance allele) and its fast-neutron-derived "Bgh-induced tip cell death1" mutant, bcd1, were compared using the 22K Barley1 GeneChip. Planting, stage of seedlings, harvest...
ORGANISM(S): Hordeum vulgare 
2009-02-25 | E-GEOD-14930 | biostudies-arrayexpress
TranscriptomicsGenetic regulation of gene expression of barley in response to stem rust (Pgt isolate TTKS)
The QxSM doubled-haploid mapping population was generated from a single Q21861 x SM89010 F1 plant (Borovkova et al. 1995; Steffenson et al. 1995). Four flats (each flat contained 75 DH lines + 4 replicates of each parent = 81 cones/flat) were grown in a completely randomized design at the ARS Cereal...
ORGANISM(S): Hordeum vulgare 
2010-11-15 | E-GEOD-20416 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of wild type and loss-of-function mutants of Mla1 and Mla6 powdery mildew resistant allele barley plants
A parallel expression profiling of wild-type and loss-of-function mutants of Mla6 and Mla1 powdery mildew resistance alleles was conducted using Barley1 GeneChip. Barley plants were inoculated with powdery mildew isolate 5874 and first leaves were harvested at 6 time points after pathogen inoculat...
ORGANISM(S): Hordeum vulgare 
2008-11-19 | E-TABM-142 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of barley plants containing variants of Mla1 and Mla6 powdery mildew resistance genes
A split-split-plot design with 144 experimental units (3 replications x 4 genotypes x 6 time points x 2 treatment types) was used to profile barley plants containing variants of Mla1 and Mla6 powdery mildew resistance genes in response to inoculation with the Blumeria graminis f. sp. hordei (Bgh) is...
ORGANISM(S): Hordeum vulgare 
2011-11-22 | E-GEOD-33392 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of barley Steptoe x Morex seedling leaf comparison
This study was to collect expression level polymorphism (ELP) data for the barley genotypes Steptoe and Morex, parents of a widely used North American mapping population. Primarily, the data collected will show the amount of variation between these genotypes in seedling leaves grown in a greenhouse...
ORGANISM(S): Hordeum vulgare 
2008-06-18 | E-GEOD-11182 | biostudies-arrayexpress
TranscriptomicsMla-specified Transcriptional Responses in Barley-Powdery Mildew Interactions
A large-scale parallel expression analysis was conducted to elucidate Mla-specified responses to powdery mildew infection using 22K Barley1 GeneChip probe arrays. Our goal was to identify genes differentially expressed in incompatible (resistant) vs. compatible (susceptible) and Mla-specified Rar1-...
ORGANISM(S): Hordeum vulgare 
2011-11-22 | E-GEOD-33396 | biostudies-arrayexpress
TranscriptomicsCross-species Detection in Barley1 GeneChip Array
This study was conducted to evaluate the efficiency of cross-species detection in Barley1 GeneChip array. We hybridized cRNA derived from first leaves of barley green seedlings (as a control), as well as the same stage of seedling leaf from representative genotypes of wheat, oat, rice, maize, and s...
ORGANISM(S): Triticum aestivum 
2011-11-22 | E-GEOD-33391 | biostudies-arrayexpress
TranscriptomicsCornChip0-Maize Pilot GeneChip Array
To define the parameters necessary to design short oligo arrays for maize (Zea mays L.), a species with particularly high nucleotide (SNP) and insertion-deletion (indel) polymorphism frequencies, gene expression was analyzed in for four maize inbred lines using a custom Affymetrix DNA array. Statist...
ORGANISM(S): Zea mays 
2010-06-11 | E-GEOD-4663 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of wheat development
The analysis of gene expression during wheat development:; Gene expression measurements were carried out on a developmental tissue; series for wild-type wheat (cv. Chinese Spring) using the Affymetrix; Wheat GeneChip. Thirteen tissues at defined developmental stages were; chosen to match the barley...
ORGANISM(S): Triticum aestivum 
2009-08-15 | E-GEOD-12508 | biostudies-arrayexpress
TranscriptomicsMalignant Germ Cell Tumors Display Common microRNA Profiles Resulting in Global Changes in Expression of mRNA Targets
Comparison of miRNA expression profiles in malignant germ cell tumors compared to non-malignant control group. Use of bioinformatic algorithm Sylamer to interrogate mRNA expression profiles from malignant germ cell tumors for enrichment or depletion of binding sites for differentially expressed miRN...
ORGANISM(S): Homo sapiens 
2010-03-14 | E-GEOD-18155 | biostudies-arrayexpress
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