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Protein quantification strategies using multiple proteases have been shown to deliver poor inter-protease accuracy in label-free mass spectrometry experiments. By utilizing six different proteases with different cleavage sites, this study explores the protease bias and its effect on accuracy and pre...
ORGANISM(S): Homo Sapiens 
2022-09-02 | PXD033574 | panorama
Mass spectrometry-based bottom-up proteomics is rapidly evolving and routinely applied in large biomedical studies. Proteases are a central component of every bottom-up proteomics experiment, digesting proteins into peptides. Trypsin has been the most widely applied protease in proteomics, due to it...
ORGANISM(S): Homo Sapiens 
2023-08-29 | PXD042450 | panorama
Higher plants encode four or five DICER-LIKE (DCL) enzymes responsible for the production of small non-coding RNAs which function in RNA interference (RNAi). Different RNAi pathways in plants effect transposon silencing, antiviral defense and endogenous gene regulation. DCL2 acts genetically redunda...
ORGANISM(S): Arabidopsis Thaliana 
2024-07-26 | PXD046163 | panorama
Mass spectrometry based on Data Independent Acquisition (DIA) strategies has developed into powerful tools suitable for precision medicine applications. Assays performed in this setting provide confident protein identification and precise quantification on an absolute scale when combined with stable...
ORGANISM(S): Homo Sapiens 
2024-07-03 | PXD037946 | panorama
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