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TranscriptomicsA modified bacterial one-hybrid system yields improved quantitative models of transcription factor specificity
We examine the use of high-throughput sequencing on binding sites recovered using a bacterial one-hybrid (B1H) system and find that improved models of transcription factor (TF) binding specificity can be obtained compared to standard methods of sequencing a small subset of the selected clones. We ca...
ORGANISM(S): synthetic construct 
2011-04-28 | E-GEOD-26767 | biostudies-arrayexpress
GenomicsOff-Target Analysis of Zinc Finger Nucleases
Zinc Finger Nucleases (ZFNs) facilitate precise editing of DNA enabling targeted genomic modifications in vivo. ZFNs have been employed to obtain genetically modified plants and animals, and cell-based therapies utilizing ZFNs are undergoing clinical trials. However, many ZFNs display dose-dependent...
ORGANISM(S): Danio rerio 
2010-08-25 | E-GEOD-23762 | biostudies-arrayexpress
TranscriptomicsExploring the DNA-Recognition Potential of Homeodomains
We utilized a bacterial selection system to isolate Engrailed variants from a randomized library that are compatible with each of the 64 possible 3’ triplet sites (i.e. TAANNN). The DNA-binding specificity of 151 representative HD variants from these populations was subsequently characterized. Ma...
ORGANISM(S): synthetic construct 
2012-02-15 | E-GEOD-35806 | biostudies-arrayexpress
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