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TranscriptomicsscRNA-seq of pluripotent stem cell derived human intestinal organoids
Here, we used single cell RNA-sequencing (scRNA-seq) to profile pluripotent stem cell derived human intestinal organoids (HIOs) grown in matrigel or a non-adhesive alginate hydrogel after 28 days of in vitro growth. Additionally, we used scRNA-seq to profile HIOs derived in the presence of Neureguli...
ORGANISM(S): Homo sapiens 
2021-05-21 | E-MTAB-10268 | biostudies-arrayexpress
TranscriptomicsscRNA-seq of pluripotent stem cell derived human intestinal organoids, adult intestine, and human fetal endoderm derived organs
Here, we used single cell RNA-sequencing (scRNA-seq) to profile pluripotent stem cell derived human definitive endoderm and intestinal organoids (HIOs) at several timepoints of in vitro growth (7, 14, and 28 days) and after in vivo growth beneath the kidney capsule of a murine host (4 and 8 wks post...
ORGANISM(S): Homo sapiens 
2021-05-21 | E-MTAB-10187 | biostudies-arrayexpress
TranscriptomicsscRNA-seq of human fetal intestinal ileum tissue
Here, we performed single cell RNA sequencing (scRNA-seq) of human fetal ileum tissue samples from 3 individual biological specimens ages 11.4, 14.4, and 18.9 weeks post conception. The data set is composed of over 16,000 cells from diverse intestinal lineages.
ORGANISM(S): Homo sapiens 
2021-02-22 | E-MTAB-9906 | biostudies-arrayexpress
TranscriptomicsscRNA-seq of human fetal intestine tissue
Here, we performed single cell RNA sequencing (scRNA-seq) of human fetal duodenal tissue samples from 8 individual biological specimens across 7-21 weeks of gestation. The data set is composed of over 37,000 cells (between 3,000-9,000 cells per time point) from diverse intestinal lineages. Lineages ...
ORGANISM(S): Homo sapiens 
2020-12-12 | E-MTAB-9489 | biostudies-arrayexpress
TranscriptomicsscRNA-seq of intestinal epithelial organoids derived from human fetal duodenum
Here, we used single-cell RNA-sequencing (scRNA-seq) to profile intestinal epithelial only organoids (also known as enteroids) from human fetal duodenum after one passage of in vitro growth. Organoids were grown in the standard 25% LWRN media with either 100 ng/ml of EGF or 1 ng/ml of EREG added.
ORGANISM(S): Homo sapiens 
2022-07-17 | E-MTAB-11912 | biostudies-arrayexpress
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