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OtherA novel NGS-based method can improve the efficiency and sensitivity of Y2H screening with cDNA library
The NGS-based Y2H screening methods have been adopted to increase the efficiency and sensitivity, while reducing the labor and experimental cost of the canonical Y2H screening. However, most of NGS-based Y2H screening methods are suitable for well-constructed ORFeomes but not cDNA libraries. Thus, w...
ORGANISM(S): Arabidopsis thaliana 
2021-07-09 | GSE179488 | GEO
GenomicsA novel NGS-based method can improve the efficiency and sensitivity of Y2H screening with cDNA library
A novel NGS-based method can improve the efficiency and sensitivity of Y2H screening with cDNA library
PRJNA744049 | ENA
Transcriptomicsrec-Y2H matrix in S. cerevisiae screening reveals a vast potential for direct protein-protein interactions among RNA binding proteins
RNA-binding proteins (RBPs) are crucial factors of post-transcriptional gene regulation and their modes of action are intensely investigated. At the center of attention are RNA motifs that guide where RBPs bind. However, sequence motifs recognized by RBPs are typically a poor predictor of RBP-RNA in...
ORGANISM(S): Saccharomyces cerevisiae 
2021-09-01 | E-MTAB-9612 | biostudies-arrayexpress
Genomics
High-throughput Y2H sequencing
PRJNA914778 | ENA
GenomicsDevelopment and application of a Ligation-based Library vs Library high- throughput Yeast Two-Hybrid (LLL-Y2H) screening system
Development and application of a Ligation-based Library vs Library high- throughput Yeast Two-Hybrid (LLL-Y2H) screening system
PRJNA359495 | ENA
OtherCSEP0219: Yeast two-hybrid next-generation interaction screening (Y2H-NGIS) to discover protein-protein interactions influencing barley powdery mildew disease resistance and susceptibility.
The barley powdery mildew effector (CSEP0219) dataset was part of a larger yeast two-hybrid (Y2H) screen to identify novel proteins involved in barley immune responses. Y2H screening was coupled with next-generation sequencing to identify and quantify interacting proteins. Individual baits were mate...
ORGANISM(S): Hordeum vulgare 
2026-10-05 | GSE299566 | GEO
OtherCSEP0174: Yeast two-hybrid next-generation interaction screening (Y2H-NGIS) to discover protein-protein interactions influencing barley powdery mildew disease resistance and susceptibility.
The barley powdery mildew effector (CSEP0174) dataset was part of a larger yeast two-hybrid (Y2H) screen to identify novel proteins involved in barley immune responses. Y2H screening was coupled with next-generation sequencing to identify and quantify interacting proteins. Individual baits were mate...
ORGANISM(S): Hordeum vulgare 
2026-10-05 | GSE299565 | GEO
OtherDevelopment and application of a Ligation-based Library vs Library high- throughput Yeast Two-Hybrid (LLL-Y2H) screening system
We developed a Ligation-based Library vs Library high-throughput Yeast Two-Hybrid (LLL-Y2H) screening system to explore protein interactions.
ORGANISM(S): Mycobacterium tuberculosis Mus musculus 
2019-12-30 | GSE93036 | GEO
Genomics
Liquid Y2H-Sequencing of GmFT1a/GmFT2a interating proteins
PRJNA714747 | ENA
UnknownNext-generation yeast-two-hybrid analysis with Y2H-SCORES identifies novel interactors of the MLA immune receptor.
Not available
S-EPMC8046355 | biostudies-literature
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