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Since we have found that the RhoC gene causes extensive changes to gene expression, a methylation array was carried out to determine if the observed transcriptional changes are due to methylation changes in the genome.
ORGANISM(S): Homo sapiens 
The aim of the experiment was to identify regions of DNA bound by RhoC and WDR5. SiHa cells overexpressing RhoC were crosslinked, digested with MNase and sonicated. The fragmented DNA that bound to RhoC and WDR5 was pulled down using RhoC and WDR5 antibodies and sequenced using the Illumina HiSeq pl...
ORGANISM(S): Homo sapiens 
Since RhoC is known to be involved in various tumour phenotypes, a transcriptome analysis was carried out with RhoC over-expressing cells to identify the genes altered by RhoC over-expression.
ORGANISM(S): Homo sapiens 
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