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HSC-2 (hepatic stellate cells line from rat) were stably transfected with rno-miR-146a. Three different clones were selected (S1, S4, S5). We used Affymetrix rat genome RAT230 2.0 chip to monitor global transcriptome changes. Two passages of each sample were used. Control - HSC-2 and GFPscr.
ORGANISM(S): Rattus norvegicus 
pHSCs were isolated from Wistar rats and cultivated in cell culture flasks. The miRNA was isolated at day 0 and 10 and hybridized onto the Ncode Multi-species miRNA microarray V2 from Invitrogen 3 samples from different rats were analyzed on miRNA microarray performing dye swap
ORGANISM(S): Rattus norvegicus 

BACKGROUND: Birthweight reflects in utero exposures and later health evolution. Despite existing studies employing high-dimensional molecular measurements, the understanding of underlying mechanisms of birthweight remains limited.

METHODS: To investigate th...

2020-06-19 | MTBLS1684 | MetaboLights
In this study, through coupling of our ISDoT tissue decellularisation technology with quantitative mass spectrometry, we explored the changing tumour matrisome during mammary tumourigenesis in the PyMT breast cancer model compared to age-matched healthy control mammary gland
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2022-03-30 | MSV000089167 | MassIVE
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