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To reveal subclass I SnRK2-interacting proteins, we conducted co-immunoprecipitation using anti-GFP antibody from subclass I SnRK2-GFP expressing plants. Immunoprecipitates were identified by LC-MS/MS analyses.
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2020-04-27 | PXD017371 | Pride
To identify genes acting downstream of MAP kinase kinase, and thus map its signal, we used the steroid-inducible overexpression system. The constitutive active form MKK3 (MKK3DD) or MKK4 (MKK4DD) was cloned into the glucocorticoid-inducible vector pTA7002 and transformed using Agrobacterium method i...
ORGANISM(S): Arabidopsis thaliana 
Wild-type A. thaliana plants (Arabidopsis thaliana L. ecotype-Columbia) were grown in plastic pots filled with peat moss for 3 weeks (principal growth stage 1.07M-^V1.08) under a 16 h light/8 h dark regimen (40 M-1 10 umol photons/m2/s) at 22C. Cold treatment: The 3-week-old plants were transferred...
ORGANISM(S): Arabidopsis thaliana 
Rice plants (Oryza sativa L. cv. Nipponbare) were grown in plastic pots filled with nutrient soil for 2 weeks under flooded lowland conditions with a 12-h light (28C)/12-h dark (25C) regimen (ca. 1500 umol photons mM-^V2 sM-^V1). The plants were cold treated by transfer from 28C to 10C with growth f...
ORGANISM(S): Oryza sativa (japonica cultivar-group) 
Rice plants (Oryza sativa L. cv. Nipponbare) were grown in plastic pots filled with nutrient soil for 2 weeks under flooded lowland conditions with a 12-h light (28C)/12-h dark (25C) regimen (ca. 1500 umol photons mM-^V2 sM-^V1). The plants were then subjected to dehydration treatment by growth at 2...
ORGANISM(S): Oryza sativa (japonica cultivar-group) 
Rice plants (Oryza sativa L. cv. Nipponbare) were grown in plastic pots filled with nutrient soil for 2 weeks under flooded lowland conditions with a 12-h light (28C)/12-h dark (25C) regimen (ca. 1500 umol photons mM-^V2 sM-^V1). The plants were cold treated by transfer from 28C to 10C with growth f...
ORGANISM(S): Oryza sativa (japonica cultivar-group) 
Wild-type A. thaliana plants (Arabidopsis thaliana L. ecotype-Columbia) were grown in plastic pots filled with peat moss for 3 weeks (principal growth stage 1.07M-^V1.08) under a 16 h light/8 h dark regimen (40 M-1 10 umol photons/m2/s) at 22C. Dehydration treatment: The 3-week-old plants were grow...
ORGANISM(S): Arabidopsis thaliana 
Rice plants (Oryza sativa L. cv. Nipponbare) were grown in plastic pots filled with nutrient soil for 2 weeks under flooded lowland conditions with a 12-h light (28C)/12-h dark (25C) regimen (ca. 1500 umol photons mM-^V2 sM-^V1). The plants were subjected to dehydration treatment by growth at 28C wi...
ORGANISM(S): Oryza sativa (japonica cultivar-group) 
We performed LC-MS/MS analysis by using recombinant proteins.
ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress) 
[to be updated] Keywords: other
ORGANISM(S): Zea mays 
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