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TranscriptomicsmiRNA expression profile in GIST (gastrointestinal stromal tumor)
To clarify aberrantly expressed miRNAs affecting the biology of GIST, miRNA array was performed in 19 cases of GIST. A series of 19 GISTs specimens were analyzed using 3D-Gene Human miRNA Oligo chips containing 904 human miRNAs and 107 viral miRNAs anti-sense probes printed in duplicate spots (Toray...
ORGANISM(S): Homo sapiens 
2013-03-01 | E-GEOD-36087 | biostudies-arrayexpress
ProteomicsLFQ of Spot-Trap IP-MS of MIC11-Spot-HA expressing Toxoplasma
∆MIC11::MIC11-Spot-HA parasites or the parental ∆MIC11 parasites were fixed with 0.1% PFA and lysed in HEPES-RIPA buffer (20 mM HEPES-NaOH, pH 7.5, 1 mM EGTA, 1 mM MgCl2, 150 mM NaCl, 0.25% sodium deoxycholate, 0.05% SDS, 1% NP-40) supplemented with protease inhibitor cocktail cOmplete EDTA-free (Ro...
ORGANISM(S): Cellular Organisms 
2026-03-04 | PXD062810 | JPOST Repository
ProteomicsLFQ of mNeonGreen-Trap IP-MS from cells expressing HKDC1-mNeonGreen
Cells stably expressing full-length HKDC1-mNeonGreen or △N20 HKDC1-mNeonGreen proteins were grown in a 10-cm dish and were treated with or without antimycin/oligomycin for 3h, and then cross-linked with 0.1% formaldehyde for 10min at room temperature. After cross-linking was quenched with 100mM glyc...
ORGANISM(S): Homo Sapiens (human) 
2024-01-21 | PXD040970 | JPOST Repository
ProteomicsLFQ of anti-HA IP-MS of PLP1-3HA expressing Toxoplasma
Control (ROP9-3HA) parasites or PLP1-3HA expressing parasites were fixed with 0.1% PFA and lysed in HEPES-RIPA buffer (20 mM HEPES-NaOH, pH 7.5, 1 mM EGTA, 1 mM MgCI2, 150 mM NaCl, 0.25% sodium deoxycholate, 0.05% SDS, 1% NP-40) supplemented with protease inhibitor cocktail cOmplete EDTA-free (Roche...
ORGANISM(S): Cellular Organisms 
2026-03-04 | PXD068211 | JPOST Repository
ProteomicsLFQ of Spot-Trap IP-MS from MEF cells expressing Irgb6-Spot
Irgb6-deficient MEFs stably expressing Spot-tagged Irgb6 were infected with or without T. gondii ME49 for 4 h and lysed in guanidine buffer (6 M guanidine-HCl, 100 mM Tris-HCl, pH 8.0, and 2 mM DTT). The lysates were diluted 8-fold with RIPA buffer (20 mM HEPES-NaOH, pH 7.5, 150 mM NaCl, 1 mM EGTA, ...
ORGANISM(S): Mus Musculus (mouse) 
2023-11-21 | PXD043421 | JPOST Repository
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