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GenomicsData Access Committee of National Institute of Genetics of Japan
Data Access Committee EGAC00001000448
EGAC00001000448 | EGA
TranscriptomicsExpression profiling of mouse CD4+ T cells following Klf1 retrovirus vector transfection
Krüppel-like factor 1 (Klf1) is a transcription factor that promotes β-globin synthesis in erythroid progenitors, and its role in immunological homeostasis is unknown. The full-length mRNA of mouse Klf1(NM-010635) was cloned into the pMIG retroviral vector, which contains an IRES-GFP cassette. Splen...
ORGANISM(S): Mus musculus 
2018-05-10 | E-MTAB-6778 | biostudies-arrayexpress
TranscriptomicsDownregulation of microRNA-132 is associated with liver metastasis of colorectal cancer by targeting Anoctamin 1.
In this study, we conducted a microarray-based analysis to identify differentially expressed miRNAs in CRC by comparing miRNA profiles among primary CRC tissues from patients with liver metastases, primary tissues without liver metastases, and liver metastatic lesions. microRNAs (miRNAs) have been s...
ORGANISM(S): Homo sapiens 
2016-02-14 | E-GEOD-72199 | biostudies-arrayexpress
TranscriptomicsIdentification of a novel biomarker serum miRNA from the patients with colorectal cancer (CRC).
Serum microRNAs (miRNAs) have been shown to have a potential for cancer diagnosis lately. The main objective of this study is to identify a novel biomarker serum miRNA from the patients with colorectal cancer (CRC). Microarray analysis of miRNA expression was performed using paired pre- and post- op...
ORGANISM(S): Homo sapiens 
2014-10-02 | E-GEOD-55139 | biostudies-arrayexpress
Genomicsena-DATASET-NIG-07-03-2016-14:49:59:413-12 - samples
We performed target re-sequencing for 1.29 Mb interval of chromosome 9 (chr9:21299764–22590271, hg19). NimbleGen SeqCap EZ choice system was used as a target enrichment method (Roche Diagnostics). A DNA probe set complementary to the target region was designed by NimbleDesign. The libraries were seq...
EGAD00001001942 | EGA
GenomicsSearching for variants associated with endometriosis
We explored regulatory mechanism of a SNP on chromosome 9p21 associated with endometriosis by leveraging “allele-specific” functional genomic approaches. By re-sequencing 1.29 Mb of 9p21 region and scrutinizing DNase-seq data from the ENCODE project, we prioritized rs17761446 as a candidate function...
EGAS00001001741 | EGA
ProteomicsAtPhot2, Autophosphorylation, Gel
Recombinant Arabidopsis phototropin2 (Phot2) protein or kinase-dead derivative (Phot2-DN) were incubated with ATP under different conditions. Proteins were separated by SDS-PAGE, in-gel tryptic digested, analyzed by LC-MS.
ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress) 
2018-10-09 | PXD007908 | JPOST Repository
UnknownComparison of the modified Collard and hand-sewn anastomosis for cervical esophagogastric anastomosis after esophagectomy in esophageal cancer patients: A propensity score-matched analysis.
Not available
S-EPMC6345657 | biostudies-literature
ProteomicsPIM kinases facilitate lentiviral evasion from SAMHD1 restriction via Vpx phosphorylation
Proteomic analysis revealed potential phospho-acceptor sites within Vpx by PIM3. HEK293 cells were transfected with plasmid vector encoding Vpx together with either empty vector or PIM3. Immunoprecipitated Vpx was subjected to liquid chromatography tandem-mass spectrometry.
ORGANISM(S): Homo Sapiens (human) 
2019-03-20 | PXD013154 | JPOST Repository
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