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TranscriptomicsExpression data from primary central nervous system lymphoma (PCNSL) patients
This study aimed to define the genes associated with PCNSL patient survival. Expression profiling was performed on 34 PCNSLs. A gene classifier was developed. Thirty-four PCNSL patients were selected for RNA extraction and hybridization on Affymetrix microarrays.
ORGANISM(S): Homo sapiens 
2012-11-06 | E-GEOD-34771 | biostudies-arrayexpress
TranscriptomicsExpression data from glioma patients
We used microarrays to select the genes associated glioma patients survival. This study aimed to define genes associated with survival. Expression profiling was performed on 50 gliomas. A gene classifier was developed. Fifty glioma patients were selected for RNA extraction and hybridization on Aff...
ORGANISM(S): Homo sapiens 
2016-01-08 | E-GEOD-43378 | biostudies-arrayexpress
TranscriptomicsGene repression with H3K27me3 modification in human small cell lung cancer
Small cell lung cancer (SCLC) is a subtype of lung cancer with poor prognosis due to early dissemination and rapid growth. We here analyze gene expression profile of 23 clinical SCLC samples. EZH2 was found to be highly expressed in SCLC samples compared to 42 normal tissues including the normal lun...
ORGANISM(S): Homo sapiens 
2013-06-12 | E-GEOD-43346 | biostudies-arrayexpress
ProteomicsTMTpro-18plex analysis of HEK293T cells transfected with DCAF siRNAs
HEK293T cells transfected with control, CUL4, COP1, DCAF3, DCAF14, or RBBP7 siRNA were lysed in 150 µL guanidine buffer. After heating and sonication, the lysates were centrifuged at 20,000 × g for 15 min at 4°C. The supernatants were recovered, and proteins (150 µg each) were purified by methanol-c...
ORGANISM(S): Homo Sapiens (human) 
2025-11-14 | PXD057876 | JPOST Repository
ProteomicsProximity biotinylation of HEK293T cells stably expressing AirID-DCAFs
HEK293T cells stably expressing AGIA-AirID-DCAF, -SKP2, and -IκBα were cultured in a 10-cm dish in biological replicates (n = 2) and treated with 10 µM MG132 for 6 h in the presence of 10 µM biotin for 24 h. The cell pellet was then lysed in 300 µL of a guanidine buffer (6 M guanidine-HCl, 100 mM HE...
ORGANISM(S): Homo Sapiens (human) 
2025-11-14 | PXD057875 | JPOST Repository
ProteomicsDIA-based quantitative proteomics of HEK293T cells treated with MLN4294
HEK293T cells were cultured in 6-well plates and treated with DMSO or 2 µM MLN4924. After 6 h (MLN4924) or 24 h (DMSO or MLN4924), the cells (n=3) were collected using 1 mL of PBS by pipetting. All samples were centrifuged at 500 × g for 3 min at 4°C, and cell pellets were lysed in 160 µL of guanidi...
ORGANISM(S): Homo Sapiens (human) 
2026-03-05 | PXD064494 | JPOST Repository
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