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ProteomicsLFQ of PROTAC-dependent biotinylated peptides in MM1.S cells expressing AirID-CRBN
MM1.S cells stably expressing AGIA-AirID-CRBN-WT were treated with DMSO, 1 µM pomalidomide, or 0.1 µM ARV-825 in the presence of 5 µM biotin and 5 µM MG132 for 6 h in three biological replicates. After tryptic digestion, biotinylated peptides were enriched with Tamavidin 2-REV and analyzed by LC-MS/...
ORGANISM(S): Homo Sapiens (human) 
2021-11-06 | PXD028763 | JPOST Repository
ProteomicsVHL-AirID expressing MM1.S cells treated with DMSO, VH032 or MZ1
MM1.S cells stably expressing VHL-AirID were treated with DMSO, VH032 or MZ1 with MG132 in three biological replicates. Cell lysates were subjected to methanol-chloroform precipitation and digested with trypsin. Biotinylated peptides were purified by Tamavidin 2-REV and identified by LC-MS/MS analys...
ORGANISM(S): Homo Sapiens (human) 
2025-09-03 | PXD067141 | JPOST Repository
ProteomicsThBD-AGIA-AirID expressing MM1.S cells treated with DMSO, Pomalidomide or ARV-825
MM1.S cells stably expressing ThBD-AGIA-AirID were treated with DMSO, Pomalidomide or ARV-825 with MG132 in three biological replicates. Cell lysates were subjected to methanol-chloroform precipitation and digested with trypsin. Biotinylated peptides were purified by Tamavidin 2-REV and identified b...
ORGANISM(S): Homo Sapiens (human) 
2025-09-03 | PXD067142 | JPOST Repository
ProteomicsThBD-AGIA-AirID expressing MM1.S cells treated with DMSO, Pomalidomide, GA-TD-3 or GA-TD-5
MM1.S cells stably expressing ThBD-AGIA-AirID were treated with DMSO, Pomalidomide, GA-TD-3 or GA-TD-5 with MG132 in three biological replicates. Cell lysates were subjected to methanol-chloroform precipitation and digested with trypsin. Biotinylated peptides were purified by Tamavidin 2-REV and ide...
ORGANISM(S): Homo Sapiens (human) 
2025-09-03 | PXD067146 | JPOST Repository
ProteomicsComparison of three enrichment methods of biotinylated peptides or proteins from AirID-CRBN-expressing MM1.S cells
MM1.S cells stably expressing AGIA-AirID-CRBN were treated with DMSO or 10 µM pomalidomide in the presence of 10 µM biotin and 5 µM MG132 for 8 h in three biological replicates. The cells were divided into three tubes and lysed. After tryptic digestion, biotinylated peptides were enriched using Tama...
ORGANISM(S): Homo Sapiens (human) 
2021-11-06 | PXD028754 | JPOST Repository
ProteomicsTMTpro-18plex analysis of MM1.S cells treated with PROTACs
MM1.S cells were treated with DMSO, 0.3 µM Po-P, 0.3 µM Le-P, 0.3 µM F-P, 0.3 µM Cl-P, or 0.3 µM F3C-P for 16 h in three biological replicates. The cells were lysed in 100 µL guanidine buffer (6 M guanidine-HCl, 100 mM HEPES-NaOH, pH 7.5, 10 mM TCEP, 40 mM chloroacetamide). After heating and sonicat...
ORGANISM(S): Homo Sapiens (human) 
2023-07-22 | PXD037178 | JPOST Repository
ProteomicsTMTpro-18plex analysis of MM1.S cells treated with thalidomide and its derivatives
MM1.S cells were treated with DMSO or 10 µM thalidomide or its derivatives for 5 h in two biological replicates. The cells were lysed in 200 µL guanidine buffer (6 M guanidine-HCl, 100 mM HEPES-NaOH, pH 7.5, 10 mM TCEP, 40 mM chloroacetamide). After heating and sonication, proteins (100 µg each) wer...
ORGANISM(S): Homo Sapiens (human) 
2023-07-22 | PXD041812 | JPOST Repository
ProteomicsNeo-substrate selectivity of IMiDs on biotinylation by AirID-CRBN
MM1.S cells stably expressing AGIA-AirID-CRBN-WT were treated with DMSO, 20 µM thalidomide, 10 µM lenalidomide, 10 µM pomalidomide or 20 µM 5-hydroxythalidomide (5HT) in the presence of 10 µM biotin and 5 µM MG132 for 8 h in three biological replicates. After tryptic digestion, biotinylated peptides...
ORGANISM(S): Homo Sapiens (human) 
2021-11-06 | PXD028755 | JPOST Repository
ProteomicsProximity labeling of proteins involved in the mechanism of excisable DD
Excisable DD construct was fused with TurboID and transduced into NIH3T3 cells. The cells were exposed to Shield-1 (1 μM) for 24 h, followed by co-treatment of Shield-1 (1 μM) and biotin (50 µM) for 24 h (biological replicates; n = 3). The cell lysates in guanidine-TCEP buffer were dissolved by heat...
ORGANISM(S): Mus Musculus (mouse) 
2024-01-27 | PXD048060 | JPOST Repository
ProteomicsProximity biotinylation of HEK293T cells stably expressing AirID-DCAFs
HEK293T cells stably expressing AGIA-AirID-DCAF, -SKP2, and -IκBα were cultured in a 10-cm dish in biological replicates (n = 2) and treated with 10 µM MG132 for 6 h in the presence of 10 µM biotin for 24 h. The cell pellet was then lysed in 300 µL of a guanidine buffer (6 M guanidine-HCl, 100 mM HE...
ORGANISM(S): Homo Sapiens (human) 
2025-11-14 | PXD057875 | JPOST Repository
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