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ProteomicsTMT-based phosphoproteomic analysis of WT and PKD2/3-deficient iNKT cells
Parental and PKD2/3 double-deficient DN32.D3 cells were treated with 10 ug/ml anti-CD3 and cross-linked with 100 ug/ml goat anti-Hamster IgG for 5 min. Cells were washed with ice-cold HEPES-saline and lysed with Guanidine-HCl buffer (6 M guanidine-HCl, 100 mM Tris-Cl pH8.0, 2 mM DTT). The lysates we...
ORGANISM(S): Mus Musculus (mouse) 
2025-09-09 | PXD059288 | JPOST Repository
TranscriptomicsComparision of expression profile between wild-type and Slc39a13 knockout osteoblasts
In order to explore molecules whose expression is controlled by Slc39a13, we investigated gene expression profiling of primary osteoblast isolated from wild-type and Slc39a13 knockout mice. Keywords: knockout vs wild-type wild-type vs Slc39a13 knockout
ORGANISM(S): Mus musculus 
2008-11-07 | E-GEOD-10555 | biostudies-arrayexpress
ProteomicsProximity biotinylation of iNKT cells expressing TurboID-Ikaros with or without TCR stimulation
Ikaros cDNA fused to TurboID was cloned into a retroviral vector and transfected into Phoenix retrovirus packaging cells using PEI MAX (Polysceinces). Culture supernatant containing retroviruses was used for infection into DN32.D3 cells. Cells were cultured in 10%FBS/DMEM which does not contain biot...
ORGANISM(S): Mus Musculus (mouse) 
2025-09-09 | PXD059289 | JPOST Repository
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