In this study, we detected the first lysine succinylome and acetylome in a vancomycin-intermediate S. aureus (VISA) strain, XN108.Furthermore,Comparative analysis of the protein succinylation and acetylation between XN108-ΔcobB and XN108-WT strain was conducted on the basis of quantitative detection...
High-content screening (HCS) has become a powerful tool in drug discovery; however, their reliance on indirect readouts and surrogate markers limits their ability to directly assess drug-protein interactions at endogenous levels, particularly in subcellular contexts. Here, we report the development ...