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Wild-type and delta-rpoE strains of H:z66 positive S. Typhi were grown respectively in 1 ml of LB broth (pH 7.0) at 37oC with shaking (200 rpm) overnight. 200 ul of each culture was then transfer-incubated in fresh 20 ml of LB broth to log phase (0.5 OD at 600 nm). Ampicillin was added in cultures t...
ORGANISM(S): Salmonella typhi GIFU10007 
Pasteurized whole cow milk (Grade A) was purchased from the supermarket. Whole cow milk was centrifuged at 3 000 g at 4°C for 30 min and the fat layer was recovered. The recovered fat was washed with PBS for 20 min at 37°C, and the resuspened mixture was centrifuged again to obtain the milk fat. The...
ORGANISM(S): Bos taurus (Bovine) 
2018-10-19 | PXD009288 | Pride
Clostridium acetobutylicum is a typical bacterium of major importance to industrial butanol production. In order to dissect the regulatory network pertaining to the industrial application of this bacterium, catabolite control protein A (CcpA) was investigated for its global function by DNA microarra...
ORGANISM(S): Clostridium acetobutylicum 
Background: Plants attenuate their responses to a variety of bacterial and fungal pathogens, leading to higher incidences of pathogen infection at night. However, little is known about the molecular mechanism responsible for the light-induced defence response; transcriptome data would likely facilit...
ORGANISM(S): Solanum lycopersicum 
Extracellular vesicles (EV) as drug delivery nanocarriers are under intense investigation. Although clinical-grade EVs have been produced on a large-scale, low yield and high production costs of natural EVs (nEV) limit the relevant industrial translation. Recent studies show that mechanical extrusio...
ORGANISM(S): Homo sapiens (Human) 
2023-03-11 | PXD030826 | Pride
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