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ProteomicsHuman Cell 293T BCAT1 IP GC-LC-MS
To identify the potential interaction protein that interacts with BCAT1 by IP-MS, we established the stable cell line with vector and BCAT1 in AGS. Then ten million cells were collected lysed in ice-cold 0.1% NP-40 buffer (50 mM Na2HPO4, pH 7.5, 150 mM NaCl) with protease inhibitors cocktail (Sigma,...
ORGANISM(S): Homo sapiens (Human) 
2024-11-15 | PXD041556 | Pride
Huamn bcat1 ip-ms 
TranscriptomicsGlobal gene expression profiles of Oryza sativa wild type Nipponbare and mutant coi1-13
To reveal the underlying molecular mechanism of jasmonate inhibits gibberellins signaling in rice, we performed transcriptional profiling of wild type nipponbare and mutant coi1-13 plants on a global scale using the Affymetrix GeneChip Rice Genome Array Rice young uppermost internodes were harvested...
ORGANISM(S): Oryza sativa 
2011-12-01 | E-GEOD-29577 | biostudies-arrayexpress
ProteomicsPHF8 Demethylates YY1 to Regulate ETC Gene and mROS to Promote Cancer Cell Growth
High levels of mitochondrial reactive oxygen species (mROS) are linked to cancer development, which is tightly controlled by electron transport chain (ETC). Targeting ETC represents a promising way of curing cancers. However, the epigenetic mechanisms governing ETC gene transcription to drive mROS p...
ORGANISM(S): Homo Sapiens 
2022-06-07 | PXD034400 |
ProteomicsTargeting high circDNA2v levels in colorectal cancer induces cellular senescence and elicits an anti-tumor secretome
The efficacy of immunotherapy against colorectal cancer (CRC) is impaired by insufficient immune cell recruitment into the tumor microenvironment. Our study shows that targeting circDNA2v, a circular RNA commonly overexpressed in CRC, can be exploited to elicit cytotoxic T-cell recruitment. CircDNA2...
ORGANISM(S): Homo Sapiens 
2024-04-11 | PXD051359 |
ProteomicsChaperonin TRiC bridges radial spokes for folding proteins locally translated in mammalian sperm flagella
As animals evolved from external to internal fertilization, sperm flagella, which once transiently propelled sperm in water to reach nearby eggs, developed to beat for days or even longer within the female reproductive tract. How flagella were remodeled accordingly remains unclear. Unlike externally...
ORGANISM(S): Bos Taurus 
2025-08-12 | PXD067693 |
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