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ProteomicsAccurate identification of proteogenomic characterization in Mycolicibacterium smegmatis MC2155 by 14N and 15N double-labeling mass spectra
To achieve deep coverage of M. smegmatis proteome, 240 μg proteins were reduced with 5 mM of dithiothreitol (DTT), alkylated with 20 mM of iodoacetamide (IAA). The alkylated proteins were separated by a 10% SDS-PAGE for 8 cm, and stained with Coomassie Blue G250. Gel lane was excised into 13 fractio...
ORGANISM(S): Mycolicibacterium Smegmatis Mc2 155 
2022-09-29 | PXD037081 |
ProteomicsAc-LysargiNase Helps Genome Reannotation of Mycolicibacterium smegmatis MC2 155
To achieve deep coverage of M. smegmatis proteome, 240 μg proteins were reduced with 5 mM of dithiothreitol (DTT), alkylated with 20 mM of iodoacetamide (IAA). The alkylated proteins were separated by a 10% SDS-PAGE for 8 cm, and stained with Coomassie Blue G250. Gel lane was excised into 13 fractio...
ORGANISM(S): Mycolicibacterium Smegmatis Mc2 155 
2022-09-29 | PXD037080 |
ProteomicsiTRAQ-based proteomic analysis of Deinococcus radiodurans in response to 12C6+ heavy ions irradiation
Proteome of Deinococcus radiodurans which were treaded by four different doses of heavy ion radiation.
ORGANISM(S): Deinococcus Radiodurans R1 
2022-02-28 | PXD031902 |
ProteomicsPhosphoproteomics attributes aberrant signaling events to a druggable kinome in hepatocellular carcinom
To connect aberrant phospho-signaling events to therapeutic vulnerabilities in hepatocellular carcinoma (HCC), we performed a mass-spectrometry-based phosphoproteomic quantification of human HCC tumors, adjacent tissues and cell lines. We developed an integrated pipeline as the inference of druggabl...
ORGANISM(S): Homo Sapiens 
2023-01-02 | PXD023840 |
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